摘要
采用微量ＤＮＡ提取技术，从梅花鹿血、毛、鹿鞭、鹿茸、牛鞭、驴鞭中提取ＤＮＡ，以线粒体ＤＮＡ细胞色素ｂ通用引物Ｌ１４８４１和Ｈ１５１４９扩增约３０７ｂｐＤＮＡ片段，扩增产物纯化后采用双脱氧链终止法测定其序列。结果证明：梅花鹿毛、血和鹿鞭的ＤＮＡ序列完全一致；而所谓的“鹿茸”则与其有较大的差异。用所测序列以简约法ＰＡＵＰ３１１程序构建的分子系统树与传统分类系统相吻合。说明此方法鉴定鹿鞭是科学而准确的。
The trace DNA extraction method was used to extract DNA from the blood,hair,Cornu cervi pantotrichum. hairy antler of the sika deer,Cervus nippon,and penis of Bulbalus bublis and Asinus vulgaris. A 307bp fragment of mitochondrial DNA cytochrome b gene was amplified with primers L14841 and H15149 for these extractions.The purified PCR products were sequenced by the dideoxy method.DNA sequence obtained from the Cornu cervi pantotrichum is the same as that obtained from the fresh blood and hair of the sika deer,but the sequence of the so called hairy antler is quite different,implying that it is not from the sika deer.In addition,the phylogenetic relationships among different species are congruent with the traditional known phylogeny.
出处
《中国中药杂志》
CAS
CSCD
北大核心
1997年第10期579-583,共5页
China Journal of Chinese Materia Medica
关键词
鹿鞭
DNA
序列测定
动物药材
中药鉴定
Cornu cervi pantotrichum
DNA sequence analysis
identification
animal medicine appraisal
