摘要
目的观察ＩＬ－２脂质体对ＣＤ３ＡＫ体内抗肿瘤的增强作用。方法：利用Ｃ５７ＢＬ／６小鼠脾细胞与抗ＣＤ３单克隆抗体和白细胞介素———２（ＩＬ－２）共同培养制备ＣＤ３ＡＫ细胞（ＣＤ３ＭｃＡｂａｃｔｉｖａｔｅｄｋｉｌｅｒｃｅｌｓ）；从脾内注射Ｂ１６黑色素瘤细胞制备鼠肝转移瘤模型：用ＰＣ和ＣＨ合成ＩＬ－２复合脂质体；ＣＤ３ＡＫ细胞联合ＩＬ－２脂质体用于体内抗Ｂ１６黑色素瘤肝转移。结果：共同培养７天时ＣＤ３ＡＫ数即扩增到原来的８倍以上，明显高于ＬＡＫ细胞（Ｐ＜００１），体外杀伤Ｂ１６黑色素瘤细胞亦强于ＬＡＫ细胞（Ｐ＜０００５）。ＣＤ３ＡＫ联合脂质体包裹的ＩＬ－２能显著减少肝转移瘤结节的数目（Ｐ＜００１），鼠外周血淋巴细胞明显增加（Ｐ＜００１）；而同等剂量的ＣＤ３ＡＫ细胞加游离ＩＬ－２、游离ＩＬ－２及空白脂质体等组均无效。结论：本实验结果指示ＣＤ３ＡＫ细胞具有强的体内扩增能力及杀瘤活性。
Aim:To observeted the antitumor efficacy of combining CD3AK cell with IL-2 Liposomes in a murine hepatic metastasis mode utilizing the B16 melanoma cells Method:The anti-CD3McAb activated killer cells(CD3AK cells) were induced by culturing C57BL/6 murine splenocytes with anti-CD3 monoclonal antibody and interleukin-2(IL-2).The B16 melanoma hepatic metastases models were established by injecting single tumor cells into spleen in C57BL/6 mice.Liposomes were prepared by using PC and CH.Result Expansion of cell numbers of CD3AK cells was 2.7 and 4.4 fold compared with LAK cells in 5 days and 7 days culture(p<0.001).Cytolytic activity of CD3AK cells was higher then LAK cells in vitro(P<0.005).The numbers of tumor hepatic metastasis were reduced significantly by combining CD3AK cells with IL-2 Liposomes in vivo(P<0.01) Conclusion:These results indicate that:(1)CD3AK cells are ease to be prepared and can generate large numbers effect cells,(2)the combination of CD3AK cells and IL-2 liposomes have synergistic antitumor efficacy in models of adoptive immunotherapy.
出处
《胃肠病学和肝病学杂志》
CAS
1997年第3期231-234,243,共5页
Chinese Journal of Gastroenterology and Hepatology
关键词
肝肿瘤
脂质体
白细胞介素2
杀伤细胞
Anti-CD3McAb,Anti-CD3McAb activated killer cells,Liposomes,Interleukin-2,Tumor hepatic metastasis
