摘要
目的研究肝癌组织中脯氨酸羟化酶(PHD)2蛋白的表达情况及其与缺氧诱导因子(HIF)-1α蛋白表达和患者临床特征的关系。方法选取肝细胞癌标本80例,应用免疫组织化学染色方法(SP法)研究PHD2蛋白与HIF-1α蛋白的表达情况,并探讨PHD2蛋白的表达与多种临床指标的关系。结果PHD2在肝癌组织中表达的阳性率为47.5%(38/80),与HIF-1α蛋白(66.3%)的表达率无显著差异,但在低分化肝细胞癌组织中的表达率明显高于高分化者,与肿瘤直径和是否有转移或者血管侵犯没有明显关系。结论PHD2虽然是HIF-1α的直接下游基因,但其蛋白表达受多种因素的影响,两者的关系在肝癌组织中没有协同性。以增加肿瘤组织中PHD2的表达降解HIF-1α来治疗肿瘤可能对低分化肿瘤的效果明显。
Objective To investigate the expression of PHD2 and HIF-1α and their clinical significance in human hepatocellular carcinoma. Methods The expression of PHD2 and HIF-1α in 80 cases of HCC were studied by immunohistochemistry assay. Results The expression of PHD2 and HIF-1α were 47. 5%（38/80） and 66. 3% respectively. There was no significant correlation between the expression of the two kinds of protein in HCC by Chi-square test. The increased expression of PHD2 protein is found in less differentiated HCC and is not associated with the diameter of carcinomas and metastasis. Conclusion Although PHD2 is the direct HIF gene, there are no significant relationship between the expression of the two kinds of proteins because there are more other factors to influence the expression of PHD2. In conclusion, it is possible to therapy HCC by adding the expression of PHD2, especially in less differentiated HCC.
出处
《华中医学杂志》
CAS
2008年第2期116-117,129,共3页
Central China Medical Journal
