摘要
目的研究新城疫病毒(NDV)对人舌鳞癌TSCCa细胞survivin表达及细胞周期的影响。方法通过NDV体外感染人舌鳞癌TSCCa细胞,MTT方法检测TSCCa细胞的生长情况;采用RT-PCR和Western bloting检测survivin表达变化;流式细胞术分析检测NDV感染后TSCCa细胞分裂周期各时相的变化及细胞凋亡情况。结果NDV感染后TSCCa细胞降低survivin表达,细胞凋亡率增加,S和G2/M期细胞减少,增殖指数(PI)降低,与阴性对照组比较有显著性差异(P<0.01)。结论NDV感染可抑制TSCCa细胞survivin的表达,影响细胞周期,诱导细胞凋亡。
Objective To investigate the effects of Newcastle disease virus （NDV） infection on the expression of survivin and cell cycle＇in human tongue squamous carcinoma TSCCa cells. Methods The proliferation of TSCCa cells infected with NDV in vitro was evaluated by means of MTT assay, and survivin expression in the infected cells was detected using RT-PCR and Western blotting. Flow cytometry was performed to assess the changes in the cell apoptosis, cell cycle and cell proliferation index （PD of the cells. Results NDV infection resulted in decreased survivin expression and increased apoptosis of TSCCa cells, with reduced cell percentage in G：CM and S phases and lowered PI of the cells, showing significant differences from those of the negative control cells （P〈0.05）. Conclusion NDV infection can inhibit survivin expression, affect the cell cycle of TSCCa cells and induce their apoptosis.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2008年第4期558-561,共4页
Journal of Southern Medical University
基金
内蒙自然基金(200408020902)
内蒙古医学院附属医院重大项目(200405)~~
