摘要
鸡胚中过高的母源抗体会干扰Lasota毒株的繁殖。本试验结果证明，当母源抗体〉2^6时种毒（EID50 10^8.5，HA 2^10）稀释1×10^3～5×10^3倍；母源抗体平均2^4左右时，种毒稀释5×10^3～1×10^4倍；母源抗体降至2^3左右时，种毒稀释倍数1×10^4～1×10^5为宜。按以上方法接种鸡胚，所收毒液效价最高。
High maternal antibody in chick embryo influenced reproduction of Newcastle Disease Virus （Lasota strain）. The result of experiment showed when the maternal antibody was over 2^6 （HI）, the seed virus（EIDs0 108.5, HA 2^10）was diluted 1×10^3 -5×10^3times, when the maternal antibody was 24, the seed virus was diluted 5×10^3 -1×10^4times, when the maternal antibody was 23, The seed virus was diluted 1 ×10^4-1×10^5times, according to this method to inoculate chick embryo, the titer of virus in embryo water was the highest.
出处
《中国兽药杂志》
2008年第4期35-36,共2页
Chinese Journal of Veterinary Drug
关键词
新城疫病毒
母源抗体
病毒繁殖
种毒稀释
效价
newcastle eisease virus
maternal antibody
breed reproduce
dilute
titer.
