摘要
以1周龄海蓝白蛋鸡为实验对象,采用连续腹腔注射的方法建立铝中毒模型,研究铝对鸡大脑组织钙稳态的影响。实验设对照、低、中、高4个剂量组,暴露剂量分别为0、18.31、27.47、36.62mg/(kg·d)(Al3+),染铝60d后,用原子吸收分光光度法检测鸡大脑组织中铝的含量,以Fura-2/AM作为细胞内游离钙离子荧光指示剂,检测鸡大脑神经细胞中游离钙离子浓度([Ca2+]i),采用半定量RT-PCR法检测鸡大脑组织中钙调蛋白(CaM)mRNA的表达。结果表明,各染铝组鸡大脑组织铝含量、神经细胞[Ca2+]i显著高于对照组(P<0.05,P<0.01),CaMmRNA表达水平显著低于对照组(P<0.05,P<0.01);且呈剂量-效应关系。提示铝暴露可致鸡大脑神经细胞内钙稳态失调。
The model chicks was produced by continuous peritoneal injection of different concen-tration aluminium trichloride to investigate the effect of aluminum on calcium homeostasis in chicken brain at doses of Al^3＋ 0,18.31,27.47 and 36,62 mg/（kg .d）for successive 60 days, The content of aluminum in brain tissues were measured by atomic absorption spectroscopy. The concentration of [Ca^2＋]i in fowl nerve cells was detected by Furoa-2/AM as the calcium fluorescent indicator, Expression of CaMmRNA was detected through semi-quantitative reverse transcriptase-polymerase chain reaction（RT-PCR）. The results showed that the content of AI and [Ca^2＋]i in the nerve cell were higher（P〈0.05, P〈0.01）and expression of CaMmRNA was lower （P〈0.05,P〈0.01）than control significantly. The results indicated that aluminum disturbed the calcium homeostasis in the nerve cell of chicken brain.
出处
《中国家禽》
北大核心
2008年第7期18-21,共4页
China Poultry
基金
黑龙江省教育厅科研项目(11521019)
