摘要
本研究采用RAPD标记对不同海拔下分布的鹅绒委陵菜6个种群进行了克隆结构、克隆多样性以及克隆多样性与海拔因子之间相关性的研究。研究结果表明,13条随机引物对120个个体进行PCR扩增,扩增到132条带,其中多态性条带117,多态位点比率(PPL)为88.64%,共检测到68个基因型,且克隆多样性水平较高,Simpson指数平均为0.875,基因型比率PD平均为0.527。鹅绒委陵菜种群内克隆之间的镶嵌明显,这可能与它的克隆构型有关。采用SPSS软件对鹅绒委陵菜种群的克隆多样性与海拔进行相关性分析,结果显示,它们之间并无显著的相关性。
This study used RAPD analysis to investigate the clonal structure, clonal diversity and relativity between clonal diversity and altitude in six P. anserine populations. Thirteen random primers were selected for amplification and 132 repetitive loci with 117 polymorphic loci were detected. The total average percentage of polymorphic loci （PPL） was 88.64% and we could differentiate 68 RAPD genotypes among 120 plants. The clonal diversity was high in the populations： The mean Simpson＇s index was 0. 875 and PD was 0. 527. The mosaic among P. anserine clones was clear,suggesting that this is probably because of its guerilla clonal structure. SPSS analysis showed that clonal diversity of P. anserine was not significantly related to altitude.
出处
《草业学报》
CSCD
2008年第2期68-74,共7页
Acta Prataculturae Sinica
基金
甘肃省高等学校研究生导师科研项目(0501-08)资助
