摘要
对纯化的北京鸭脂蛋白进行了电镜观察、梯度凝胶电泳、总胆固醇及卵磷脂胆固醇脂酰转移酶(LCAT)活力分布测定等研究 ,对鸭apoAⅠ的氨基酸组成、序列、亲 疏水性同人及其他动物进行了比较并初步进行了二级结构预测 .实验结果进一步证实北京鸭血清胆固醇是由高密度脂蛋白 (HDL)携带运输 。
The purified Beijing duck serum lipoproteins were observed by electronmicroscope for its shape and size. The distributions of total cholesterol and LCAT activities and their gradient gel elctrophoresis, the amino acid composition and partial amino acid sequences of each fragment of apo A-I were studied and determined, respectively. Furthermore, their hydrophilicity and hydrophobicity of α-helices were also studied and compared with human and other species. When the above results were integrated together they provided further evidence for the hypothesis that the cholesterol of Beijing duck is carried and transported by HDL instead of LDL and apo A-I played an important role in the cholesterol metabolism.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1997年第4期334-339,共6页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金资助项目! (392 70 76 4)
