摘要
目的：探讨溶酶体相关4次跨膜蛋白质B（lysosome-associated protein transmembrane 4 beta，LAPTM4B）基因多态性与肝癌易感性的关系．方法：应用病例对照研究方法，收集190例肝癌患者、190例慢性乙型肝炎患者、175例健康献血者全血，分离白细胞，提取基因组DNA，采用特异性引物PCR方法，扩增LAPTM4B第一外显子5′UTR内的部分序列，对三组人群进行分析研究．x^2检验分析肝癌组与对照组LAPTM4B的基因多态性和其他相关因素的相关性．结果：LAPTM4B等位基因在三组观察对象中的分布，^＊1和^＊2在健康对照组的频率分别是75．71％和24．29％，慢肝组73．16％和26、84％，肝癌组中66．84％和33.45％，三组比较等位基因分布频率有统计学意义，健康对照组与肝癌组比较有统计学意义（x^2=6．979，P=0．008）．LAPTM4B的基因型LAPTM4B1^＊1型、LAPTM4B^＊1／2混合型和LAPTM482^＊2型在肝癌组中的频率分别是37.9％、57.9％和4．2％、慢肝组50．5％、45．3％和4．2％、健康对照组56．6％、38.3％和5．1％，三组间三种基因型分布频率比较有统计学意义（x^2=14．854，P〈0．005）.结论：基因型^＊1／2和等位基因^＊2可能与肝癌的发生有关．
AIM： To investigate the possible association between allelic variation of lysosome-associated protein transmembrane 4β （LAPTM4β） and genetic susceptibility to liver cancer.
METHODS： Genotype of LAPTM4β was detected in 190 patients with liver cancer, 175 healthy adults and 190 chronic hepatitis B （CHB） patients by special primers-based polymerase chain reaction. Genotypic distribution of LAPTM4β was analyzed by x^2 test. RESULTS： The allelic frequency of ^＊1 and ^＊2 was 68.8%, 33.3%, 73.21%, 26.8%, 75.7% and 24.29% in the of HCC, chronic hepatitis B and healthy control groups, which was significantly different between the HCC and healthy control groups （x^2 = 6.979, P = 0.008）. The risk of suffering from HCC was increased 1.547-fold. The LAPTM4β genotypes ^＊1^＊1,^＊2^＊2, and ^＊1^＊2 were 37.9%, 57.9% and 4.2% in the HCC group, 50.5%, 45.3% and 4.2% in the CHB group and 56.6%,38.3% and 5.1% in the health control group （x^2 = 14.854, P 〈 0.005）.
CONCLUSION： The allele of LAPTM4β is associated with the genotypic susceptibility to liver cancer.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第8期908-911,共4页
World Chinese Journal of Digestology
关键词
肝癌易感性
基因多态性
乙型肝炎
溶酶体相关4次跨膜蛋白质B
Susceptibility to liver cancer
Polymorphism gene, Hepatitis B
Lysosome-associated protein transmembrane 4 beta
