摘要
目的:探讨TGF-β1和TGF-βR1蛋白及其前体mRNA的表达与胃癌发生发展的关系.方法:采用免疫组化和实时PCR(real-time PCR)方法,对胃癌50例、萎缩性胃炎19例和正常胃黏膜18例的TGF-β1和TGF-βR1蛋白及其前体mRNA的表达进行检测.结果:胃癌组织中TGF-β1和TGF-βR1蛋白表达明显增强,其阳性率(80.0%和75.0%)明显高于正常胃黏膜组(33.3%和27.8%)及萎缩性胃炎组(36.8%和36.8%),差异有显著性(P<0.01).胃癌组织的分化程度越低,TGF-β1、TGF-βR1蛋白表达的阳性率越高(r=35.58,P<0.01).同样,胃癌组织中TGF-β1和TGF-βR1前体mRNA的表达明显高于萎缩性胃炎组(TGF-β1:4.20±0.51 vs 9.15±2.12,8.22±1.81;TGF-βR1:1.28±0.48 vs 5.55±1.48,4.19±0.95).结论:TGF-β1和TGF-βR1的高表达与胃癌的发生发展、生物学行为和预后可能有关.
AIM： To evaluate the significance of transforming growth factor-β1 （TGF-β1） and its β 1 type receptor （TGF-βR1） in the pathogenesis of gastric carcinoma.
METHODS： TGF-β1 and TGF-β R1 were detected by immunohistochemistry in and real-time PCR （RT-PCR） 50 cases of gastric carcinoma, 19 cases of atrophic gastritis and 18 cases of normal gastric mucosa.
RESULTS： Immunohistochemistry demonstrated that the positive expression rates of TGF-β1 and TGF-β R1 were significantly higher in gastric carcinoma （80.0% and 75.0%） than in normal gastric mucosa （33.3 % and 27.8%） and atrophic gastritis （36.8 % and 36.8%） （P 〈 0.01）. The lower the differentiation of gastric carcinoma was, the higher the expression of TGF-β1 and TGF-β R1 was （r = 35.58, P 〈 0.01）. Furthermore, the expression of TGF-β1 and TGF-β R1 mRNA was significantly higher in gastric carcinoma than in normal gastric mucosa and atrophic gastritis （TGF-β1： 4.20 ±0.51 vs 9.15 ±2.12, 8.22 ± 1.81; TGF-β RI： 1.28±0.48 vs 5.55 ±1.48, 4.19 ± 0.95）.
CONCLUSION： High expression of TGF-β1 and TGF-β R1 is correlated with the development, biologic behavior and prognosis of gastric carcinoma.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第8期856-861,共6页
World Chinese Journal of Digestology
基金
国家自然科学基金
No.30470642
No.30670780
青岛市科技局资助项目
No.05-1-JC-93
山东省卫生厅资助项目
No.鲁卫科教国合函(2007)37~~
