摘要
将N-乙烯基吡咯烷酮(NVP)接枝聚合到硅胶表面,制得接枝微粒PVP/SiO2;使接枝微粒PVP/SiO2在乙醇溶液中与碘发生络合反应,形成水不溶的聚维酮碘PVP-I2/SiO2,即实现了聚维酮碘的固载化.考察了各种因素对络合反应的影响规律,用红外光谱与化学分析法对功能微粒PVP-I2/SiO2的化学结构与组成进行了表征.以大肠杆菌为致病菌体,采用平板活菌计数法研究了PVP-I2/SiO2的杀菌性能.研究结果表明,PVP/SiO2与碘的络合反应的适宜温度为60℃,经12h后达到络合平衡,络合度随溶液中碘浓度的增大而提高,最大约为0.16%(ω).固载化的功能微粒PVP-I2/SiO2具有很强的杀菌能力,在药剂量为5g/L的条件下,与浓度为109CFU/mL的菌悬液接触3min,即可使杀菌率达100%;胞外DNA和RNA测定与TTC-脱氢酶活性测定结果验证了水不溶聚维酮碘PVP-I2/SiO2的杀菌机理。
N-vinyl pyrrolidone （NVP） was grafted on silica gel particles, resulting in the grafted particles of poly（pyrrolidone）/SiO2 （PVP/SiO2）. The complexation reaction between PVP/SiO2 and iodine was conducted in ethanol as solvent. The immobilization of povidone-iodine on SiO2 was realized, and the functional particles of PVP-I2/SiO2 were obtained. The effects of various influential factors on the complexation reaction were examined. The structure and composition of the functionalized particles of PVP-I2/SiO2 were characterized with Fourier transform infrared spectrum and chemical analysis method. The sterilization property of PVP-I2/SiO2 was studied with Escherichia coil as a disease-leading bacterium and the method of live bacteria plate counting. The experimental results show that the suitable temperature for the complexation reaction is 60 ℃, and the complexation degree increases with the increase of iodine concentration. At 60 ℃ a maximum complexation degree of 0.16%（ω） is obtained as iodine is dissolved in ethanol in saturated state. The PVP-I2/SiO2 particles have strong sterilization activity. Their sterilization rate can reach 100% as they contact with the cell suspension, which has a concentration of 1×10^9 CFU/mL, only at a dosage of 5 g/L and for 3 min. TTC-dehydrogenase determination and measurement of extracellular DNA and RNA have fully verified the sterilization mechanism of PVP-I2/SiO2.
出处
《过程工程学报》
EI
CAS
CSCD
北大核心
2008年第2期339-344,共6页
The Chinese Journal of Process Engineering
基金
山西省自然科学基金资助项目(编号:MZ20020402)
关键词
聚维酮碘
接枝聚合
络合反应
水不溶杀菌材料
杀菌机理
povidone-iodine
grafting polymerization
complexation reaction
water insoluble antibacterial material
sterilization mechanism
