摘要
目的探讨神经颗粒素在系统性红斑狼疮（SLE）患者和正常人外周血单个核细胞（PBMCs）的表达状态。方法对比分析SLEPBMCs基因表达系列分析（SAGE）文库的高表达标签，并采用反转录聚合酶链反应（RT-PCR）法检测与正常人群有明显差异表达的神经颗粒素在SLE患者PBMCs的mRNA表达水平。结果对比分析SLEPBMCs和正常人各类淋巴细胞SAGE文库显示：神经颗粒素标签仅异位高表达于SLE患者，而几乎不表达于正常人各类淋巴细胞。RT-PCR检测证实：活动组SLE患者神经颗粒素mRNA表达水平较正常对照明显增加（p〈0．001），而缓解组SLE患者其表达水平仅稍有增加（P〉0．05）。结论神经颗粒素作为一种前凋亡因子，高表达于SLEPBMCs，可能介导或参与SLE异常的免疫调节反应。
Objective To investigate the mRNA expression level of neurogranin on peripheral blood mononuclear cells （PBMCs） from patients with systemic lupus erythematosus （SLE）. Methods Top 20 tags of SLE PBMCs SAGE library were searched from normal lymphocytes SAGE library including navie-T, Th1, Th2, CD8^＋ T, NK and B cells, and their abundance was compared. The mRNA expression level of neurogranin, a differential over-expressed tag, was detected in 35 cases of SLE and 15 normal controls by reverse transcription- polymerase chain reaction （RT- PCR）. Results Neurogranin tag could only be detected in SLE PBMCs SAGE library, but was hardly found in normal lymphocyte SAGE library. However, either SLE patients or normal controls showed a detectable mRNA level of neurogranin on PBMCs by RT- PCR. The mRNA level of neurogranin in active SLE patients was significantly increased than those in controls （P〈0.001）, but only slightly increased in inactive SLE patients （P〉0.05）. Conclusion Neurogranin , as a novel proapototic factor, is overexpressed on PBMCs of SLE patients. It may be involved in the regulation of abnormal immune responses in lupus.
出处
《中华风湿病学杂志》
CAS
CSCD
2008年第4期265-268,共4页
Chinese Journal of Rheumatology
