摘要
目的:研究清肺消咳颗粒的药效学作用。方法:采用氨水引咳、酚红祛痰、整体平喘与离体气管条收缩试验评价镇咳、祛痰、平喘作用;采用体外抗菌及病原菌感染小鼠模型,细胞病变法(CPE)、噻唑蓝法(MTT)及流感病毒感染动物模型,二甲苯致小鼠耳廓肿胀与大鼠棉球肉芽肿炎性模型,评价抑菌、抗病毒与抗炎作用。结果:清肺消咳颗粒能明显抑制浓氨水诱导的小鼠咳嗽,可显著增加小鼠酚红分泌量,对"组胺-乙酰胆碱"诱发的豚鼠哮喘有明显的缓解作用,可显著延长豚鼠呼吸困难与引喘潜伏期。清肺消咳颗粒对肺炎双球菌、甲/乙型溶血性链球菌、金黄色葡萄球菌均有较明显的抑制或灭活作用,对大肠杆菌和绿脓杆菌的抑制作用较弱,可提高肺炎双球菌感染小鼠的生存率和存活时间;清肺消咳颗粒在12.5 mg·mL-1浓度下对MDCK细胞无毒性,在0.78 mg·mL-1浓度时即可有效抑制流感病毒的毒性,对流感病毒诱发小鼠肺炎的肺指数有明显的降低作用;清肺消咳颗粒可显著抑制二甲苯致小鼠耳廓肿胀和大鼠棉球致肉芽组织增生。结论:清肺消咳颗粒有明显的镇咳、祛痰、平喘作用和抑菌、抗病毒、抗炎作用。
Objective ： To study effects of Qingfeixiaoke granule on antitussive, expectorant, antiasthmatic and anti-bacteria, anti-virus, anti-inflammatory. Methods：The following experiments were carried out：cough induced by ammonia, removing phlegm with phenol red, in vivo and in vitro tests for relieving asthma. The effects of the drug on relieving cough, removing phlegm and relieving asthma were investigated;The in-vitro anti-bacteria, and the mouse model infected by pathogenic bacteria, The mouse model of ear edema caused by xylene and the nat midle of tampon granuloma were established to evaluate the anti-bacteria, anti-virus, anti-inflammatory effects. Results：The antitussive expectorant and antiasthmatic experiments of Qingfeixiaoke granule showed that it can restrain mice to cough by ammonia, increase phenol red secretion of mice in bronchus, release asthma induced by histamine phosphate-acetylcholine in guinea pigs, increased latent period of breathe with difficulty and induced asthma, the Anti-bacteria, anti-virus and anti-inflammatory experiments showed that Qingfeixiaoke granule had strong an effect on inhibiting or drowned activity pneumonia streptococcus,α-β hemolytic streptococcus, staphylococcus aureus and had infirm an effect on inhibiting bacillus coli and bacillus pyocyaneus, the death rate of infected by pneumonia streptococcus in mice could reduce and could prolong the mean survival days. Qingfeixiaoke granule indicated no toxicity to MDCK cells at 12.5 mg·mL^-1, and could effectively inhibit influenza A1 virus at 0.78 mg·mL^- 1, could markedly debase indexes of lung of mouse pneumonitis which was elicited by influenza virus; Qingfeixiaoke granule could significantly suppress ear swell induced by xylene in mice and granuloma proliferation. Conclusions： Qingfeixiaoke granule has significant effects on relieving cough, removing phlegm, relieving asthma, also shown effects on anti-bacteria, anti-virus, anti-inflammatory.
出处
《中国实验方剂学杂志》
CAS
2008年第1期33-38,共6页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
清肺消咳颗粒
镇咳
祛痰
平喘
抑菌
抗病毒
抗炎
Qingfeixiaoke Granule
antitussive
expectorant
antiasthmatic
anti-bacteria
anti-virus
anti-inflammatory
