摘要
目的:研究survivin基因表达沉默后对PC-3细胞的裸鼠体内成瘤率,瘤体生长速度,以及平均瘤重等方面产生的影响。方法:使用RNA干涉技术,用构建的pSilencer 3.1-SVV1、pSilencer 3.1-SVV2、pSilenc-er3.1-SVV3 siRNA表达质粒和阴性对照质粒pSilencer3.1-NC转染PC-3细胞,使PC-3细胞的survivin基因表达沉默,随后将细胞接种于裸鼠皮下,观察转染了各质粒的PC-3细胞在裸鼠体内成瘤率,瘤体生长速度,以及平均瘤重等方面的变化。结果:pSilencer 3.1-SVV2和pSilencer 3.1-SVV3质粒转染组的裸鼠肿瘤成瘤率明显下降,裸鼠瘤体生长速度明显慢于未转染组和pSilencer 3.1-NC质粒转染组(P<0.01)。实验终止时,肿瘤平均体积与瘤重与未转染组和pSilencer 3.1-NC质粒转染组相比减少50%-55%。结论:RNAi介导的survivin基因沉默可显著影响PC-3细胞裸鼠体内成瘤能力及瘤体生长速度。
Objective： To suppress the tumor formation and grown of PC - 3 cells in nude mice xenografts by silencing of survivin by RNAi. Methods ： Forty nude mices were received subcutaneously injection of 0.5ml （2.0 × 10^7/ml） PC - 3 cells which transfected with pSilencer3.1 - SVV1, pSilencer3.1 - SVV2 and pSilencer 3.1 - SVV3 and pSileneer3.1 - H1 neo negative control vectors respectively. The tumors volume were measured every day and weight were rffeasured after 4 weeks. The expression of survivin in tumors was confirmed by immunohistoehemistry. Results： After 4 weeks, the mean volume and weight of tumors which transfeeted with pSileneer3.1 - SVV2 and pSilencer3.1 - SVV3 vectors were less than 50% - 55% contrast with control groups. The immunohistoehemical staining of tumours showed that the expression of survivin was not different between five groups. Conclusion： The tumor formation and grown in nude mice xenografts were suppressed by silencing of survivin inhibition of survivin gene expression in prostate cancer cells by RNAi may provide novel approaches for gene therapy on androgen - independent prostate
出处
《现代肿瘤医学》
CAS
2008年第4期506-510,共5页
Journal of Modern Oncology
