摘要
目的探讨小剂量氯化镉对颌下腺颗粒曲管（GCT）细胞线粒体的损伤及丙酸睾酮的保护作用。方法35只雄性Wistar大鼠分为3组：对照（C）组、镉（Cd）组和镉加丙酸睾酮（CA＋T）组。利用透射电镜作颌下腺GCT细胞线粒体的超微结构观察，并利用image-plus-pro全自动图像分析系统作线粒体损伤的定量研究。结果镉注射后24h,GCT细胞内出现不同程度的线粒体肿胀和（或）空泡化，胞质和线粒体内可见髓样结构，7～15d后上述改变明显加重，30d后细胞形态仍未恢复正常。Cd＋T组的超微结构损伤较相应CA组轻。CA组在镉注射后3～15d，Gcr细胞线粒体的各项指标与对照组相比均有显著差异（P〈0．05）。CA＋T组仅在镉注射后7d和15d，GCT细胞线粒体比表面显著低于对照组（P〈．05）。结论镉对GCT细胞线粒体的超微结构有损伤作用，补充雄激素对上述损伤有保护作用。
Objective To Investigate the impaired effects of low-dose cadmium chloride and the protective effects of testosterone propionate on mitochondria of CJCT cells in submandibular gland of male rats. Methods Thirty-five male adult Wistar rats were randomly divided into three groups： control group, Cd injecting group and Cd ＋ T injecting group. The morphologic changes of GCT cells were observeded by transmission electron microscopy, and the quantitative analysis were performed on mitochondria with image-plus-pro analysis system. Results The mainly ultrastructural changes after injecting Cd in 24 hours included swelling and/or vacuolation of mitochondria, increasing of myelin figures in cytoplasm and mitochondria of GCT cells, which were most prominent in 7-15d, moreover, the changed morphology of GCT cells didn＇t recover in 30d. On the other hand, the ultrastructural lesions of GCT cells in Cd＋T group were gentler compared with Cd group. In image analysis, there was significant difference between Cd group and control group in 3- 15d （P;0.05）, and between Cd＋T group and control group in 7d and 15d （P;0. 05）. Conclusions The present study indicate that the ultrastructure of mitochondria will be injured by cadmium. Furthermore, supplying testosterone can prevent the mitochondria in GCT cells from toxicity of cadmium.
出处
《四川解剖学杂志》
2008年第1期10-12,共3页
Sichuan Journal of Anatomy
关键词
颌下腺
镉
丙酸睾酮
超微结构
Submandibular gland
Cadmium
Testosterone propionate
Ultrastructure
