摘要
目的探讨使用纳米羟基磷灰石(Nano-Hydroxyapitite,n-HA)作为骨组织工程支架材料的可行性。方法以成骨条件培养液培养兔骨髓基质细胞(BMSCs)至第3代并以1×106/cm2的密度与n-HA复合培养后回植于骨缺损区(1.0cm×0.8cm×0.5cm)。术后6周、12周处死动物,取缺损区及周围骨质进行肉眼、X线片、免疫组化染色观察及计算机图像分析。结果所有植入物无排除或感染。6周及12周通过肉眼及免疫组化观察实验组修复效果明显优于对照组,新骨面积百分比高于对照组(P<0.05),差异有统计学意义。结论n-HA作为骨组织工程支架材料具有可行性。
Objective To observe the feasibility of Nano- Hydroxyapitite（n-HA）as tissue engineering bone material. Melhods BMSCs from the adult rabbit（n = 6） marrow were cultured in vitro and induced to be osteoblast.The third generation BMSCs were combined with the n- HA and then implanted into the autologous furcation bone defects （1.0 cm × 0.8 cm × 0.85 cm）. At 6 or 12 weeks, the rabbits were sacri- riced. The specimens were processed for gross inspection, computer image analysis and immunohistochemical observation, respectively. Re- suits The results of the experiment group were better than those of the control group, and the trabeculation of bone in the experiment group was earlier than that of the control group. The area in new bone in BMSCs-n-HA group was obviously higher than that of control group （ P 〈 0.05）. Conclusion It is practicable to use Nano-Hydroxyapitite as tissue engineering bone material.
出处
《口腔医学》
CAS
2008年第3期154-156,共3页
Stomatology
关键词
骨髓基质细胞
羟基磷灰石
成骨细胞
骨缺损
组织工程
兔
bone marrow stromal cells
Hydroxyapitite
osteoblast
bone density
tissue engineering
rabbits
