摘要
本研究观察了二氧化氮（ＮＯ２）对大鼠肺脂质过氧化、抗氧化酶及花生四烯酸代谢的影响。实验选用Ｗｉｓｔａｒ雄性健康成年大鼠，体重１５０～１７５ｇ，设计浓度：自然空气，０．１５，１．５，１５ｍｇ／ｍ３ＮＯ２组。暴露方法：静式染毒，每天２小时，每周６天，染毒４周。实验结果表明：（１）随着ＮＯ２浓度的递增，大鼠尿中血栓烷素Ｂ２与６－酮－前列腺素Ｆ１α比值逐渐升高，且存在剂量－反应关系。（２）１．５ｍｇ／ｍ３ＮＯ２能致大鼠肺丙二醛含量增加，同时可见谷胱甘肽过氧化物酶活性升高，且两者相关具有高度显著性（Ｐ＜０．０１）。这表明：大鼠肺能通过某些抗氧化酶活性的升高而免受脂质过氧化物的毒害。（３）１．５ｍｇ／ｍ３ＮＯ２能致大鼠肺血栓烷素Ｂ２增加，６－酮－前列腺素Ｆ１α降低。同时可见丙二醛与６－酮－前列腺素Ｆ１α之间存在显著负相关（Ｐ＜０．０５）。经对尿中血栓烷素Ｂ２，６－酮－前列腺素Ｆ１α及其比值与肺中相应指标作相关分析，各指标相关具有显著性。表明：测定尿中血栓烷素Ｂ２与６－酮－前列腺素Ｆ１α含量的变化能反映ＮＯ２对大鼠肺花生四烯酸代谢的影响，用它作为一个能反映ＮＯ２健康效应、灵敏的生化指标成为可能。
The effects of NO 2 on rats were studied in vivo,by measurement of molnodialdehyde(MDA),glutathione peroxidase(GSH PX),superoxide dismutase(SOD),6 keto prostaglandine F 1(6 keto PGF 1),thromane B 2(TXB 2).We choiced specitic pathogen free male wistar rats,weighting 150￣175g,exposed to either room air or 0.15,1.5,15mg/m 3 NO 2 in an 250L exposure chamber for 4 weeks,2 hours/per day,6 days/per week.Our results showed that 1.5,15mg/m 3 NO 2 could elevated significantly ratios of urinary TXA 2/Keto PGF 1α .It indicated that there was dose effect relationship between NO 2 and urinary AA metabolites.In the experiment of effects of 1.5mg/m 3 NO 2 on rat′s lungs,our results showed that NO 2 could significantly increased the amounts of MDA and GSH PX activities in lung homogenates on 4th,7th day respectively it showed a significant positive correlationship between MDA and GSP PX( P <0.01).It is possible that the rats could defend lung cells against cytotoxic lipid peroxide by increaseing some antioxidative protective enzymes,such as GSH PX and also found that the contents of TXB 2 increased on 7th and the contents of 6 keto PGF 1 deseased significantly on 4th,7th,14th.We found that it showed a significant negative correlationship between MDA and 6 keto PGF 1α ( P < 0.05 ).Comparing the pulmonary TXB 2,6 keto PGF 1α with urine′s,there is significant positive correlationship( P < 0.05). This showed that changes of urinary TXB 2,6 keto PGF 1α may reflect the effects of NO 2 on AA metabolism of lung well,and be as a good,early and sensitive biochemical indicator for healthy effects.
出处
《环境与健康杂志》
CAS
CSSCI
CSCD
北大核心
1997年第1期4-7,共4页
Journal of Environment and Health
基金
国家自然科学基金
