摘要
目的:探讨组织蛋白酶B(cathepsin B,CB)及其组织抑制剂(cystatin C,CC)在卵巢肿瘤组织中的表达,及其与卵巢恶性肿瘤临床病理特征和预后的关系。方法:应用RT-PCR方法检测47例卵巢恶性肿瘤、21例良性卵巢肿瘤和20例正常卵巢组织中CB及CC的表达情况,进行阳性率及相对表达含量的比较,并将其结果与临床病理学资料进行统计学分析。结果:CB在卵巢恶性肿瘤中的表达水平(0.8792±0.82081)明显高于良性肿瘤中的表达水平(0.3911±0.32814),P=0.006。其相对表达含量与腹水量有关。CC卵巢恶性肿瘤中的表达水平(1.5306±1.84967)明显高于良性肿瘤中的表达水平(0.6903±0.50520),P=0.018。其相对表达含量在病理分级、肝转移、大网膜转移以及腹水方面,差异均有统计学意义,P<0.05。多因素生存回归分析显示,CB可以作为卵巢癌预后的独立因素。结论:CB及CC可能与卵巢癌的侵袭转移有关。
OBJECTIVE： To investigate the expressions of cathepsin B and its inhibitor （cystatin C） in ovarian carcinoma and explore their correlations with the clinicopathologic characteristics of patients with ovarian carcinoma. METHODS：The tissue specimens were divided into three groups： normal ovary （20）, benign neoplasm （21）, and ovarian carcinoma （47） groups. Reverse transcription polymerase chain reaction （RT-PCR） was performed to detect the expressions of cathepsin B and cystatin C mRNA. RESULTS： The semi-quantity of cathepsin B mRNA in ovarian malignant tumor was significantly higher than that in benign neoplasm tissue （P= 0. 006）. The semi-quantity of cystatin C mRNA in ovarian malignant tumor was significantly higher than that in benign neoplasm tissue （P=0. 018）. Cathepsin B correlated with ascites. Cystatin C correlated with the grade, liver metastasis, colic omentum metastasis and ascites. Multivariate regression survival analysis showed that cathepsin B could predict the malignancy status. CONCLUSION： The result provides the convincing evidence that cathepsin B and cystatin C may contribute to the mechanisms of invasion and metastatsis of ovarian carcinoma.
出处
《中华肿瘤防治杂志》
CAS
2008年第5期363-366,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
广西壮族自治区卫生厅重点课题(重200614)
