摘要
目的:探讨钟状晚期牙胚和成熟牙及牙周组织的特殊染色应用。方法:钟状晚期的人牙胚和成年大鼠的牙及牙周组织石蜡切片,用改良的Gomori和Mallory染色法染色,镜检染色结果。结果:改良Gomori染色,可将钟状晚期牙胚中的釉质基质与牙本质基质、牙及牙周组织中的硬组织与软组织、骨与软骨、染成2种不同的颜色,便于识别和区分。各种组织结构和细胞形态清晰。结论:牙发育和牙及牙周组织教学科研切片,用改良的Gomori染色,利于观察研究,取得满意结果。
Objective:To study the application of Gomori special staining in the late bell stage of dental germ,tooth and periodontium.Methods:Paraffin-embedded sections of human dental germ in late bell stage,tooth and periodontium of mature rats were stained by improved Gomori and Mallory special staining method.Results were observed by light microscope.Results:Improved Gomori special staining method can easily discriminate enamel matrix and dentin matrix in late bell stage of dental germ,sclerous tissues and soft tissues in mature tooth and periodontium,bone and cartilage in two different colouration.Cells and tissues can be observed clearly.Conclusion:Dental germ,tooth and periodontium can be stained by improved Gomori special staining with satisfactory results.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2008年第2期213-215,共3页
Journal of Practical Stomatology
基金
国家自然科学基金(编号:30471893)
关键词
牙
牙胚
牙周组织
特殊染色
Tooth
Dental germ
Periodontium
Special staining