摘要
In order to evaluate the cytocompatibility and biocompatibility of a new kind of chitosan blend film as a carrier of corneal endothelial cell, rabbit corneal endothelial cells cultured in vitro were breeded onto the film. After a cell monolayer formed, the scanning electron micrography was performed. After inplanted into anterior chamber, slit lamp observation, thickness metering, specular microscopy and HE staining were performed at random time after operation to evaluate the biocompatibility. Inflmmnation in anterior, thickness of cornea, cell density, hexagonality and cell size of the surgical cornea were taken as the indexes of biocompatibility. The cultured cells exhibited a confluent monolayer 10 days after incubation, which proved the satisfactory cytocompatibility of this film. Biocompatibility assay results suggested the implantation feasibility of the film as a carder of corneal endothelial cells.
In order to evaluate the cytocompatibility and biocompatibility of a new kind of chitosan blend film asa carrier of corneal endothelial cell,rabbit corneal endothelial cells cultured in vitro were breeded ontothe film.After a cell monolayer formed,the scanning electron micrography was performed.After inplant-ed into anterior chamber,slit lamp observation,thickness metering,specular microscopy and HE stainingwere performed at random time after operation to evaluate the bioeompatibility.Inflammation in anterior,thickness of comea,cell density,hexagonality and cell size of the surgical cornea were taken as the in-dexes of biocompatibility.The cultured cells exhibited a confluent monolayer 10 days after incubation,which proved the satisfactory eytoeompatibility of this film.Bioeompatibility assay results suggested theimplantation feasibility of the film as a carrier of corneal endothelial cells.
基金
the High Technology Research and Development Program of China(2003AA625050)
the National Natural Science Foundation of China(30070220)
