摘要
应用酶联免疫吸附试验（ＥＬＩＳＡ）对１０４例育龄妇女的血清进行了巨细胞病毒（ＨＣＭＶ）ＩｇＧ、ＩｇＭ抗体的检测，同时用ＥＬＩＳＡ双抗体夹心法测定了不同感染状态下血清中可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）的水平，并将ｓＩＬ－２Ｒ水平与未感染ＨＣＭＶ的正常育龄妇女进行了比较。结果，育龄妇女中抗－ＨＣＭＶＩｇＧ的阳性率为８９．４％，ＩｇＭ的阳性率为９．６％，感染ＨＣＭＶ的妇女血清中ｓＩＬ－２Ｒ水平均大于未感染的对照组（１７８．１±５７．３Ｕ／ｍｌ），Ｐ＜０．０５，其中ＩｇＭ阳性者和ＩｇＭ、ＩｇＧ同时阳性者血清中ｓＩＬ－２Ｒ水平最高，分别为９１０±４６５．６Ｕ／ｍｌ和９０５±３４７．８Ｕ／ｍｌ，两者间的差异无显著性意义（Ｐ＞０．０５），但均大于仅抗－ＨＣＭＶＩｇＧ阳性者（４４６．８±１５８．９Ｕ／ｍｌ），Ｐ均＜０．０５。表明，ＨＣＭＶ感染可致ｓＩＬ－２Ｒ水平升高，并且活动性感染者上升明显。提示：ｓＩＬ－２Ｒ可能参与了ＨＣＭＶ的免疫致病机制。
In order to study the relationship betwee human cytomegalovirus infection and soluble interleukin 2 receptor (sIL-2R) level, 104 serum samples were selected from women of childbearing age in the city of Wuhan and examined with ELISA for anti HCMV antibody IgG and IgM. Then we detected the sIL-2R level with double antibody ELISA in these sera with different conditions of HCMV infection. The results of different groups were compared with those women without cytomegalovirus infection and also compared with each other. The positive rate for anti HCMV antibody IgG in the women of Wuhan was 89.4% (93/104) and for anti HCMV antibody IgM only was 9.6% (10/104). The sIL-2R level of these women with HCMV infection was significantly higher than that in the control group (178.1+57.3 U/ml), P>0.05. The highest level of sIL-2R were in those women whose anti HCMV antibody IgM were positive (910+465.6 U/ml) and seeondly was in those with anti HCMV antibody IgG, IgM positive (905±347.8U/ml). They were both higer than that in those women only with anti HCMV antibody IgG positive (446.8+158.9 U/ml), P>0.05. The results showed that HCMV infection can raise the sIL-2R level in serum of childbearing age women, especially in those with active HCMV infection. sIL-2R may be one of the pathogenic factors of HCMV.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1997年第3期256-258,共3页
Chinese Journal of Experimental and Clinical Virology
