摘要
研究深低温停循环（ＤＨＣＡ）与逆行脑灌注（ＲＣＰ）时脑组织自由基的变化。健康杂种犬１４只，随机等分为ＤＨＣＡ组和ＲＣＰ组，在停循环前（Ａ点）、ＤＨＣＡ／ＲＣＰ３０分（Ｂ点）、ＤＨＣＡ／ＲＣＰ６０分（Ｃ点）、ＤＨＣＡ／ＲＣＰ９０分（Ｄ点）和复温再灌注３０分（Ｅ点）取脑皮质１ｇ，检测丙二醛（ＭＤＡ）和超氧化物歧化酶（ＳＯＤ）水平。结果见两组在Ａ点ＭＤＡ和ＳＯＤ无差别。在Ｂ、Ｃ、Ｄ、Ｅ点，ＤＨＣＡ组ＭＤＡ水平逐渐升高，ＳＯＤ含量逐渐下降；ＲＣＰ组无明显变化。两组各点比较均Ｐ＜０．００１。故认为（１）脑缺血后自由基含量增高是ＤＨＣＡ脑损伤的重要机制；（２）ＲＣＰ可维持ＤＨＣＡ时脑组织的血液供应，减轻以至消除自由基反应，减少脑损伤的危险。
Aim:To assess the effect of retrograde cerebral perfusion(RCP) on brain protection during deep hypothermic circulation arrest (DHCA).Method:Fourteen dogs were randomly divided into control group (n=7) and experimental group (n=7).DHCA was employed in both control and experimental groups.RCP was performed only in experimental group.Specimens were obtained from the cerebral tissue to measure MDA and SOD at five different time intervals:before DHCA,30,60 and 90 minutes after DHCA+RCP,and 30 minutes after reperfusion.Result:Before DHCA,no significant difference,in the levels of MDA and SOD was found between two groups.After DHCA+RCP,however,the MDA level in control group was gradually increased and SOD decreased,but there was no significant change in experimental group.At the same time intervals,there was significant difference between two groups.Conclusion:Oxygen free radical is an important factor in brain damage after DHCA.RCP could be a new approach to maintain blood supply to brain tissue during DHCA,so asto minimize or eliminate free radical reaction and reduce the risk of brain injury.
出处
《中华胸心血管外科杂志》
CSCD
北大核心
1997年第5期312-314,共3页
Chinese Journal of Thoracic and Cardiovascular Surgery
关键词
深低温停循环
逆行脑灌注
自由基
脑损伤
Deep hypothermic circulation arrest Retrograde cerebral perfusion Free radical
