摘要
为了提高云芝菌发酵生产漆酶的效率,提出了一种利用自絮凝菌丝球在三相鼓泡塔生物反应器中重复分批发酵产漆酶的新方法。在优化后的产酶条件下,考察维生素C的添加浓度对漆酶活力的影响,并通过在培养基中添加维生素C进行漆酶多批次培养。研究结果表明,维生素C的添加浓度为1.50mmol/L时,可使漆酶活力提高2.6倍。连续进行了10批培养,每批最大漆酶的活力均在1000U/ml以上,最高酶活出现在第5批为1919.6U/ml,总培养时间达25d。此方法所得漆酶对染料靛蓝具有明显的脱色降解作用,在介体1-羟基苯并三唑(HBT)用量0.10%,漆酶用量100U/L条件下作用40min时,靛蓝脱色率达到96.7%。该方法采用的三相鼓泡塔生物反应器性能稳定、菌丝球可重复使用,该方法有利于漆酶的高效、规模化生产。
To improve the laccase fermentation productivity of Coriolus vericolor, a novel method for laccase fermentation production was proposed in a three-phase bubble column bioreactor with the mycelial pellets under repeated batch process. Under the optimum laccase production conditions, the effects of the added ascorbic acid concentration on laccase activity were investigated and laccase was cultured by adding ascorbic acid in culture medium under repeated batch process. When 1.50mmol/L ascorbic acid was added to the medium, the laccase activity of fermentation broth reached 1557.9 U/ml, which was 1.6 times higher than that of the control （ no ascorbic acid was added）. Under the certain condition, laccase was produced repeatedly in 10 batches for 25 days in the repeated batch culture in the bubble column. The maximum laccase activity was 1919.6 U/ml in the fifth batch. In each batch , the maximum laccase activity were all more than 1000 U/ml. Laccase produced by the method decolorizes indigo blue markedly. The decolorization rate reached 96.7% with 1-hydroxybenzotriazole （HBT） as mediator for 40 rain, HBT concentration 0.10% and laccase dosage 100 U/l. The performance of the three-phase bubble column bioreactor is stable, and the mycelial pellets can be used repeatedly to effectively yield laccase by the method in large scale.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2008年第3期95-99,共5页
China Biotechnology
关键词
三相鼓泡塔生物反应器
云芝菌
漆酶
靛蓝
染整废水
脱色
Three-phase bubble column bioreactor Coriolus vericolor Laccase Indigo blue Dyeing wastewater Decolorization
