摘要
该文应用胶质银染技术、自动图像分析仪DNA含量测量技术对56例大肠癌手术标本进行了检测。结果发现Ag－NOR计数与患者存活时间呈负相关（r＝－0．77P＜0．001）。Ag－NOR计数低，患者存活时间长，预后好；反之则预后差。对数秩时序检验P＜0．001。在DNA含量总体直方图中，大肠癌细胞DNA主峰位于2～6C之间，预后好组，其主峰左移至2C附近，大于5C细胞群较少（10．48±9．89），而预后差组主峰右移至5C附近，大于5C细胞所占比例明显增多（61．59±21．45）。2C细胞群为主大肠癌Ag－NOR计数（4．04±0．66）低于以非整倍体细胞群占多数大肠癌（5．92±1．49）（P＜0．01）。结合运用上述指标可提高判断预后的准确性。
Ag - NOR counts and DNA content were investigated in tissue slides of 56 cases of colorectal carcinoma. There was a negative correlation between Ag - NOR counts and the patients survival time (r = - 0. 77, P< 0. 001). The differences were statistically significant using univariate chi-squares for the log rank test (x = 27. 2, P < 0.001 ). In the DNA histogram of all cases, the major peak was located from 2c to 6c. In the patients with good clinical out-comes, the major DNA peak turned left near 2c, while in the patients with poor clinical courses the major DNA peak turned right near 5c. Aneuploidy cell population in group Ⅱ (61. 59±21. 45) was significantly higher than that in group Ⅰ (10. 48±9. 89). Ag - NOR counts of the patients with predominantly diploidy cell population (4.04±0.66) were obviously lower than those of the patients with predominantly aneuploidy (5. 92±1.49, P<0.01). Com-bining the above parameters, the accuracy of the prognosis assessment will be improved.
出处
《中国现代医学杂志》
CAS
CSCD
1997年第9期9-11,共3页
China Journal of Modern Medicine
关键词
DNA
AG-NOR
预后
大肠肿瘤
Prognosis
Colorectal carcinoma
DNA content
Nucleolar organizer region
