摘要
目的探索外周血自然杀伤T(NKT)细胞比例变化与非酒精性脂肪性肝病(NAFLD)的关系。方法应用流式细胞术检测60例NAFLD患者和60名年龄、性别配对的正常对照者外周血NKT细胞比例,同时比较两组间各项临床变量的差异并分析各项临床变量与外周血NKT细胞比例变化的关系.Logistic回归分析筛选NAFLD发病的危险因素。结果正常对照组外周血NKT细胞比例的中位数及四分位数区间为1.48%(1.23%～1.98%),NAFLD组为1.12%(0.87%～1.54%),两组差异有统计学意义(P<0.001)。Spearman相关分析发现外周血NKT细胞比例与体重指数、腹围、丙氨酸转氨酶水平呈负相关(r值分别为-0.322、-0.237、-0.217:P值分别为0.001、0.021、0.035)。Logistic回归分析发现外周血NKT细胞比例、体重指数、天冬氨酸转氨酶、空腹血糖与NAFLD的发病危险因素有关(OR值分别为0.107、2.991、1.148和3.133.P值分别为0.023、0.003、0.026和0.013)。结论NAFLD患者外周血NKT细胞比例是下降的,且该比例下降可能是NAFLD发病的危险因素之一。
Objective To investigate the correlation of changes in peripheral natural killer T（NKT） cells and fatty liver disease（NAFLD）.Methods Peripheral blood samples were obtained from 60 NAFLD patients and 60 age and gender matched healthy controls. The frequency of peripheral NKT cells was detected by flow cytometry. The differences of clinical presentation and laboratory data in two groups were analyzed . The risk factors of NAFLD were screened by Logistic regression analysis. Results NAFLD patients had lower frequency of peripheral NKT cells than healthy controls [median frequencywas 1.12%（IQR0.87%-1.54%） in patients vs. 1. 48%（1. 23%-1. 98%） in controls, P〈0. 001 ]. Frequency of peripheral NKT cells was negatively correlated with body mass index（r = -0. 322, P = 0. 001）, waist circumference（r = -0, 237, P = 0. 021） and alanine aminotransferance level（r = -0. 217, P = 0. 035）. Logistic regression analysis revealed that elevated body mass index（OR = 2. 991, P = 0. 003）, aspartate aminotransferase level（OR = 1. 148, P = 0. 026）, and fasting blood sugar（OR = 3. 133, P = 0. 013） increased the risk of NAFLD, whilst elevated frequency of peripheral NKT cells（OR = 0. 107, P = 0. 023） decreased the risk. Conclusions Changes in frequency of peripheral NKT cells are correlated with NAFLD and the decreasing frequency of peripheral NKT cells might be a risk factor for NAFLD.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2007年第12期824-827,共4页
Chinese Journal of Digestion
关键词
脂肪肝
杀伤细胞
自然
危险因素
Fatty liver
Killer cells, natural
Risk factors
