摘要
有四种分子水平的生物检测标志物在当前研究环境因素-基因变异-疾病之间相关性的分子生物学领域中起着重要的作用,它们是生物大分子样品的DNA加合物、蛋白质加合物、8-羟基脱氧鸟苷、生物排泄物中的烷基化脱氧嘌呤.因为DNA的特殊作用,DNA加合物作为生物标志物倍受重视.DNA加合物的分析方法有荧光法(灵敏度达1加合物/10^(5-6)正常核酸)、免疫法(灵敏度达1加合物/10^(6-8)正常核酸)、^(32)后标记法(灵敏度达1加合物/10^(8-10)正常核酸)、HPLC+^(32)P后标记法等,由于^(32)P后标记法灵敏度高,应用范围广,是目前测生物样品DNA加合物的首选方法.
DNA adduct, protein adduct, 8-hytroxy-deoxyguanosine and alkey-deoxypurine are very important as biomarkers in human biomonitoring and study of environmental geno-toxic chemicals and health. Recently, several analytical methods have been used detect DNA adduct) it is fluorescence assays (1 adduct/105-6 normal nucleotides), immunoas-says (1 adduct/106-8 normal nucleotides), 32P-postlabeling (1 adduct/108-10 normal nucleotides), HPLC+32P-postlabiling, 32P-postlabeling assays is most noted for its sensitivity and applicability for diverse of chemicals.
出处
《环境化学》
CAS
CSCD
北大核心
1997年第2期163-166,共4页
Environmental Chemistry
