摘要
目的：建立高效液相色谱法测定山楂合剂中枸橼酸的含量。方法：采用Agilent C18色谱柱（ZORBAX SB—C184．6mm×250mm，5μm）；流动相为0．5％NH1H2PO1水溶液（用磷酸调节pH至2．8）；检测波长为210nm，流速为1．0mL／min，柱温为25℃。结果：枸橼酸进样量在0．058～29．0μg（r=0．99999）范围内与峰面积线性关系良好，平均加样回收率为99．29％，RSD=0．56％（n=9）。结论：本法简便、快速，专属性强、重现性好；可作为山楂合剂的质量控制方法。
Objective: To establish a HPLC method for the determination of citric acid in Shanzha Oral Liquid.Methods: Agilent C18 column(ZORBAX SB-C184.6 mm×250mm,5μm) was used.The mobile phase was 0.5%NH4H2PO4 solution (pH was adjusted to 2.8 by phosphoric acid).Detective wavelength was 210nm.The flow rate was 1.0mL/min.The column temperature was 25℃.Results The sample size of citric acid at the range of 0.058～29.0μg,(r=0.99999) had a good linear relation with peak area.The average recovery rate was 99.29%,RSD=0.56%(n=9).Conclusion: The method is convenient and accurate with strong exclusive and good reappearance and can be used in the quality control of citric acid in Shanzha Oral Liquid.
出处
《云南中医中药杂志》
2008年第3期43-44,共2页
Yunnan Journal of Traditional Chinese Medicine and Materia Medica
