摘要
目的探讨冠状动脉粥样硬化性心脏病(简称冠心病)患者血白细胞分化抗原14(CD14)基因C(260)T多态性与血清高敏C反应蛋白(Hs-CRP)水平的关系。方法对240例冠心病患者应用聚合酶链反应-限制性片段长度多态性(PCR-RELP)技术检测CD14基因多态性并测定Hs-CRP水平,分析两者之间的关系。结果240例冠心病患者CD14基因C(260)T多态性:呈TT基因型106例(44.2%)、TC基因型82例(34.1%)、CC基因型52例(21.7%)。Hs-CRP水平:TT基因型患者Hs-CRP(4.5±2.1)mg/L与TC基因型患者[(2.7±1.2)mg/L]及CC基因型患者[(2.9±1.1)mg/L]比较差异有统计学意义(t=10.10、8.85,均P<0.05);Hs-CRP>3.0mg/L的TT基因型高危患者占79.2%,与TC基因型高危患者(50.0%)及CC基因型高危患者(36.5%)比较,差异有统计学意义(χ2=17.75、28.04,均P<0.05)。结论T等位基因纯合子可能是冠心病炎症反应的独立影响因素,CD14基因变异可能与冠心病炎症反应有关。
Objective To investigate the relationship of serum levels of high sensitivity C-reactive protein （Hs-CRP） with CD14 C260T promoter polymorphism in patients with coronary artery disease （CAD）. Methods Serum levels of Hs-CRP were determined in 240 patients with coronary artery disease. C260T Promoter Polymorphism of the CD14 gone was screened by PCR-RELP assay. Results The genotype distribution of the C260T polymorphism of the CD14 gone was as follows.TT in 106 of 240 patients （44.2%）,TC in 82 of 240 patients （34.1%） and CC in 52 of 240 patients （21.7%）. Patients with TT subtype had increased Hs-CRP levels compared with carriers of the C allele （P〈0.05）. A higher percentage of T allele homozygotes had Hs-CRP levels〉3 mg/L （P〈0.05）. Conclusion This study indicated T allele homozygotes of CD14 gone may be an independent risk factor in systemic inflammation with stable CAD.
出处
《浙江医学》
CAS
2008年第2期110-112,共3页
Zhejiang Medical Journal
