葛根素对局灶性脑缺血大鼠转化生长因子-β_1的影响被引量：3

Effect of Puerarin on TGF-β_1 Cytokines Expression in Focal Cerebral Ischemia

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摘要目的研究大鼠缺血性脑损伤后缺血区脑组织转化生长因子-β1(TGF-β1)表达的影响,探讨葛根素对缺血性脑损伤的神经保护机制。方法108只雄性SD大鼠,用线拴法建立大脑中动脉缺血再灌注模型,随机分为手术加葛根素组、手术组、假手术组。每组又分5个不同时段观察点(6h、12h、24h、48h、72h),手术加葛根素组大鼠应用葛根素干预治疗,假手术组和手术组大鼠给予同等容量生理盐水对照,用SABC法免疫组织化学染色观察各时间点TGF-β1的表达,测算72h后大鼠脑梗死体积百分比。结果与假手术组相比,手术组和手术加葛根素组各时间点缺血区脑组织TGF-β1蛋白表达均显著增加(P<0.01)。手术加葛根素组各时间点TGF-β1的表达均较手术组显著增加(P<0.05或P<0.01)。结论葛根素注射液可能通过上调TGF-β1的表达,抑制过度的炎症反应来发挥神经保护作用。 Objective To study the nerve protective mechanism of puerarin on ischemic brain injured by the expression of TGF-β1 cytokines. Methods Models of brain medium- sized artery focal cerebral ischemia in one hundred and eight healthy male SD （Spraque- Dawley） rats were built with the improvement Zea- Longar patching me：thods. They were divided randomly into three groups： The sham operation group （ n = 36） ; the operation group （ n = 36） and the operation with puerarin interfered group （ n = 36）. There were five different observing time in every group （6 h, 12 h,24 13,48 h and 72 h）. The rats in operation with puerarin group were treated with puerarin solution. The rats in sham operation and operation group were injected with the same volume sodium chloride. The expression of TGF-β1 cytokines in different time was observed with the method of SABA immunity histochemistry staining in ischemic region. Results ① Compared with the sham operation group, there were more expression of the TNF-α, TGF-β1 cytokines in cerebral ischemic reperfusion brain tissue in the operation and the operation with puerarin interfered groups （P 〈 0.01）. ② Compared with the operation group, the expression of TNF-α cytokine was significantly decreased, while the expression of TGF-β1 cytokine was improved rapidly in different time after cerebral ischemicreperfusion in the operation with puerarin interfered group（P 〈 0.05 or P〈 0.01 ）. Conclusion Puerarin could upregulate the level of the TGF-β1 cytokine and restrained the over inflammatory reaction to protect the nerve tissue.

作者张平朱斌李彤袁彬

机构地区新乡医学院第一附属医院

出处《中西医结合心脑血管病杂志》 2008年第3期297-299,共3页 Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

关键词葛根素脑缺血转化生长因子-Β1 Puerarin cerebral isehemia TGF-β1

分类号 R285 [医药卫生—中药学]

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