摘要
为解决小血管吻合术后保证其通畅率的问题，作者将可溶性支架在含Ａｄｖ５－ＣＭＶ／ＬａｃＺ质粒的高浓度葡萄糖溶液中浸泡半小时，然后置于血管腔内进行吻合，行β－半乳糖苷酶（ＬａｃＺ基因的表达产物）活性测定和组织化学染色。结果表明这种新的在体血管转基因方法能使外源性基因在血管壁内获得有效表达，从而为小血管吻合术后血栓形成和再狭窄的基因治疗提供了理论依据。
We assessed the possibility of gene transfer into anastomotic arteries in vivo using soluble stent containing Adv5 CMV/LacZ. After being soaked in a high concentration solution of glucose containing Adv5 CMV (control group) or Adv5 CMV/LacZ (treatment group) for 30 minutes, we inserted soluble stents into the lumina of cut rat carotid arteries, and end to end anastomoses of cut rat carotid were performed with standard microvascular surgical technique. 16 rats were killed after two weeks,the segments of anastomotic carotid arteries were prepared for assessing β galactosidase activity and histochemical staining.In the control group, the anastomotic arteries did not have detectable level of β galactosidase expression. In the treatment group, the amount of β galactosidase expression was 9 80×10 3u/g tissue.Microscopic examination of histochemically stained arteries demonstrated that gene transfered not only to endothelial cells but also to smooth muscle cells, and all anastomotic arteries were transfered in the treatment group,but none of arteries revealed blue in the control group.The results of this experimental study suggested that soluble stent be a new method of direct gene transfer into arteries in vivo.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1997年第5期310-312,共3页
Chinese Journal of Surgery
