摘要
目的观察肝硬变大鼠血清及食管组织中一氧化氮（ＮＯ）含量及血流动力学改变．方法用６０％ＣＣｌ４油性溶液皮下注射，诱导肝硬变大鼠模型．随机取８只肝硬变大鼠作为实验组，另取８只正常ＳＤ大鼠作为对照组．应用５７Ｃｏ同位素标记微球技术测定肝硬变大鼠平均动脉压（ＭＡＰ）、门静脉压（ＰＶＰ）、心输出量（ＣＯ）、心脏指数（ＣＩ）、内脏血管阻力（ＳＶＲ）及内脏器官血流量（ＳＢＦ）；应用荧光比色法检测肝硬变大鼠血清及食管组织匀浆中ＮＯ含量．结果肝硬变大鼠血清（μｍｏｌ／Ｌ）及食管组织中ＮＯ含量（ｎｍｏｌ／ｇ）显著高于对照组（４２０４±１２５３对０５３２±０２５７，Ｐ＜００１；０２０３±００３２３对００６４±００１３５，Ｐ＜００１）；肝硬变大鼠全部出现高动力循环状态，其ＭＡＰ（ｋＰａ），１００ｇ体重的ＳＶＲ（ｋＰａ·ｍｌ－１·ｍｉｎ－１）显著低于对照组（１４４２±０４７对１７０５±０３４，Ｐ＜００１；２９７４±０１８６对４２３４±０１１８，Ｐ＜００１）；而ＰＶＰ，ＣＩ，ＣＯ，ＳＢＦ则显著高于对照组（Ｐ＜００１）．结论肝硬变大鼠存在高动力循环状态；内源性ＮＯ在肝硬变大鼠血流动力学改变中可能?
AIM To investigate the changes of nitric oxide (NO) concentration in esophageal tissues or serum and hemodynamics in cirrhotic rats. METHODS Cirrhosis model was induced in male SD rats by injection of 60% CCl 4 oily solution subcutaneously. There were 8 cirrhotic rats in experimental group and 8 normal SD rats served as controls. Mean arterial pressure (MAP), portal vein pressure (PVP), cardiac output (CO), cardiac index(CI), splanchnic vascular resistance (SVR) and splanchnic blood flow(SBF) were determined using 57 Co_labeld microsphere technique. In addition, concentration of nitric oxide in esophageal tissues or serum were also measured using a fluorometric assay. RESULTS NO concentration of serum (μmol/L) or esophageal tissues (nmol/g) in cirrhotic rats were significantly higher in comparison with controls (4 204±1 253 vs 0 532±0 257, P <0 01;0 203±0 0323 vs 0 064±0 0135, P <0 01). There was a hyperdynamic circulatory state in rats with cirrhosis. MAP (kPa), SVR(kPa·ml -1 ·min -1 /100g BW) of cirrhotic rats were markedly lower than those of normal rats (14 42±0 47 vs 17 05±0 34, P <0 01;2 974±0 186 vs 4 234±0 118, P <0 01). In addition, cirrhotic rats had significantly higher PVP, CO, CI and SBF than controls( P <0 01). CONCLUSION There is a hyperdynamic circulatory state in rats with cirrhosis. The endogenous NO may play an important role in the changes of hemodynamics pattern in cirrhosis. NO may facilitate development and rupture of esophageal varices.
关键词
肝硬变
食管
一氧化氮
代谢
血流动力学
liver cirrhosis/metabolism
esophagus/chemistry
nitric oxide/metabolism
hemodynamics
