摘要
目的:探讨乙肝病毒血清标志物EIA测定法与定量定性PCR测定结果的关系。方法:从临床标本筛选300例HBsAg阳性血清,100例HBsAg阴性血清,50例抗HBs阳性血清,并采用定量定性PCR方法对其分别进行检测。结果:HBsAg阳性血清定性PCR阳性率为29.3％,定量PCR阳性率为56.0％,HBsAg阴性血清定性PCR阳性率为2.0％,定量PCR阳性率为8.0％,抗HBs阳性血清定性PCR阳性率为2.0％,定量PCR阳性率为12.0％。结论:PCR检测乙肝病毒的方法比血清标志物EIA法有更高的临床检出率,定量PCR比定性PCR有更高的临床检出率。
Aim: In order to investigate the relationship of the detective results between EIA and quantative PCR、qualitative PCR. Methods: 300 HBsAg positive samples, 100 HBsAg negstive samples and 50 Anti-HBs positive samples were chosen and detected by the methods of quantative and qulitative PCR. Results: In HBsAg postive sera, the positive ratios of qualitative PCR was 29. 3% , the postive ratios of qualitative PCR was 56. 0% , In HBsAg negative sera, the postive ratios of qualitative PCR was 2. 0% , the positive ratios of quantative PCR was 8.0% ; In Anti-HBs positive samples, the positive ratios of qualitative PCR was 2. 0% , the positive rstios of quantative PCR was 12. 0%. Conclusions: The Clinic positive ratios of PCR was higher than which of EIA, meanwhile, the clinic positive ratios of quantative was higherthan which of qualitative PCR.
出处
《中西医结合肝病杂志》
CAS
1997年第3期138-140,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
