摘要
目的:探讨地氯雷他定对慢性应激大鼠肠道肥大细胞的变化的影响。方法:将雄性SD大鼠30只随机分为对照组、慢性应激组、慢性应激+地氯雷他定组,每组10只,采用长期中等强度应激造成慢性应激大鼠模型,观察各组大鼠的行为学变化及肠道敏感性的变化并用激光共聚焦显微镜观察各组大鼠肠道的肥大细胞的变化。结果:慢性应激组比对照组表现出明显的焦虑样行为、肠道运动增强、肠道敏感性增加、肠道肥大细胞数目增加(P<0.05);地氯雷他定干预组比慢性应激组的焦虑样行为、肠道运动和肠道敏感性均明显减轻,同时肠道肥大细胞数目减少(P<0.05)。结论:慢性应激大鼠肠道敏感性增加;地氯雷他定能能通过控制肥大细胞的活动减轻慢性应激大鼠肠道高敏感。
Objective： To study the influence of desloratadine on rat enter-mast cells. Methods： Thirty SD male rats were divided into three groups randomly： control group, stress group and stress＋ desloratadin group. Rats in stress group and stress＋desloratadine group were treated with chronic unpredicted mild stress. Open-field method was used to observe the change of the fecal pellet and rat behaviors （crossover and rearing）. At the end of the experiment, with the method of rectal balloon distention, the perception thresholds and the number of abdominal withdrawal reflexes （AWR） of different balloon volume were observed as well as the effects of desloratadine on the numbers and the degranulation of rat mast cells. Results： The stress group rats had more conspicuous anxiety-like behaviors than the control group did （P〈0.05）, but the behaviors seemed less in the stress＋ desloratadin group than in stress group （P〈0.05）. The perception threshold of the stress and stress＋desloratadin group was lower than that of control group （both P〈0.05）. The number of AWR to the balloon content （1.0 ml or 2.0 ml） was higher in the stress group than that in the control group （P〈0.05）, while was also higher than that in the stress＋desloratadin group. The average fluorescence intensity of mast cells protein-1 （mcp-1） in the stress group was less than that in control group and in the stress group （both P〈0.05）. Conclusion： Chronic stress is associated colonic mast cells. with increased Desloratadine rectal motor activity, anxietyqike can decrease the number of mast which may abirritate visceral hypersensitivity of chronic stress rats. behaviors, and cells in chronic numbers of stress rats.
出处
《武汉大学学报（医学版）》
CAS
2008年第2期218-221,226,共5页
Medical Journal of Wuhan University
