摘要
提出了一种检测酶反应活性中间体的动力学新方法．利用中间体的反应活性，加入合适的竞争剂使中间体在转化为产物的同时能与竞争剂反应．监测中间体与竞争剂反应的动力学过程以获得中间体信息．该法既能研究自由基中间体，也能研究非自由基中间体，便于研究快反应和跟踪动态过程．用该法对辣根过氧化物酶催化过氧化氢氧化去甲肾上腺素的中间过程进行了研究．
A new kinetic method is proposed for detecting active intermediate in enzyme reaction. Due to the reactivity of the intermediate, a proper competition reagent which reactswith the intermediate while the intermediate changes to final product, can be found andadded. By detecting the klnetic process of intermediate reaction with reagent, the information of intermediate can be obtained. The method can be used to study free radical intermediate and non-free-radical intermediate, to study fast reaction and to follow transient process.With this method, the intermediate process in oxidation reaction of norepinephrine with hydrogen peroxide, catalyzed by horseradish peroxidase, is investigated.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1997年第3期364-367,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
中间体
辣根过氧化物酶
酶反应
动力学分析
Kinetic method,Intermediate,Horseradish peroxidase,Norepinephrine
