摘要
用＾３Ｈ－天门冬氨酸为底物，林生山黧豆（ＬａｔｈｙｒｕｓｓｙｌｖｅｓｔｒｉｓＬ．）叶片匀浆上清液为粗酶液，进行体外反应。结果表明，天门冬氨酸的放射性掺入到２，４－二氨基丁酸，加入谷氨酸则能抑制这种上掺入，将上述粗酶液透析，加入可能的辅助因子，天门冬氨酸的放射性掺也到２，４－二氨基丁酸，研究证实在体外天门冬氨酸可以作为２，４－二氨基丁酸合成的底物，在林生山黧豆体内存在的催化天门冬氨酸转变为２。
An in vitro synthetic reaction system was established with 2,3-3H-aspartic acid (Asp) as a substrate and the homogenate of flatpea (Lathyrus sylvestris L.) leaves as the crude enzyme extract. The results showed that 3H-Asp was incorporated into 2,4-diaminobutyric acid (DABA). The incorporation was inhibited by the addition of glutamic acid (Glu). 3H-Asp was also incorporated into DABA after the crude enzyme was dialyzed, indicating that Asp as a substrate for DABA synthesis was catalyzed by a group of enzymes which converted Asp to DABA in flatpea. From the in vitro reactions it was proved that DABA and y-aminobutyric acid (GABA) could not be mutually substituted as substrates.
基金
国家自然科学基金
关键词
2
4-二氨基丁酸
合成
代谢
林生山黧豆
2,4-diaminobutyric acid, Anabolism, 3H-aspartic acid, r-aminobutyric acid, Lath-yrus sylvestris
