摘要
目的观察氨基胍对腹膜间皮细胞醛糖还原酶活性的影响,探讨氨基胍对腹膜间皮细胞醛糖还原酶的调节作用。方法采用人的网膜作为细胞来源,胰蛋白酶-EDTA消化法进行人腹膜间皮细胞的分离、培养,间接免疫荧光法对第二代细胞进行鉴定;用生长期细胞进行实验,培养6d后测AR活性;应用紫外分光光度计记录NADPH在340nm处的吸光值测定醛糖还原酶活性。结果实验组氨基胍组醛糖还原酶的活性与对照组比较仅略下降,无显著性差异。结论氨基胍并不能明显地抑制腹膜间皮细胞醛糖还原酶的活性。
Objective To observe the effect of aminoguanidine on activity of aldose reductase in peritoneal mesothelial cells and explore its regulation. Methods Mesothelial cells were isolated from the omental tissue of consenting patients by typsin-EDTA disaggregation and indirect immunofluorescence was used for identification. Confluent HPMCs were maintained in M199 medium supplemented with 10%FBS, 1.5% GS and 10mmol/L aminoguanidine for 6 days. Aldose reductase activity was measured on spectrophotometer by monitoring the absorbance of NADPH at 340nm. Results When HPMC was incubated with glucose in the presence of aminoguanidine（10mmol/L） for 6 days, there was a decrease in aldose reductase activity,but the difference compared to control was not significant. Conclusion Aminoguanidine can not significantly inhibit aldose reductase activity of peritoneal mesothelial cells.
出处
《中国现代医生》
2008年第7期49-50,共2页
China Modern Doctor
关键词
氨基胍
腹膜间皮细胞
醛糖还原酶
多元醇通路
Aminoguanidine
Peritoneal mesothelial cells
Aldose reductase
Polyol pathway
