摘要
目的观察脉冲电磁场（pulsed electromagnetic fields，PEMF）对5-氮胞苷（5-aza）诱导大鼠骨髓间充质干细胞（mesenchymal stem cells，MSCs）向心肌样细胞分化时细胞增殖的影响。方法50Hz脉冲低频电磁场刺激5-aza诱导后7d后的MSCs，根据不同的感应强度分为A、B、C组，每组根据作用时间的不同分为4个亚组；设未暴露PEMFs的细胞为对照组。用MTT法测定细胞增殖变化，用流式细胞技术检测细胞周期。结果0．5mT和1mT的感应强度下20～30min细胞比对照组有显著性增殖，5mT作用30～60min以及1mT作用60min与对照组相比较呈显著性抑制。流式检测细胞周期显示0．5mT和1mT在20～30min内可以引起细胞S＋G2％增高，各感应强度下作用60min以及5mT感应强度下作用20min以上均引起S＋G2％的下降。结论50Hz脉冲低频电磁场0．5mT和1mT的感应强度下，作用20～30min可通过引起诱导后的MSCs周期中S＋G2％增高而促进它的增殖。
Objective To investigate the effects of pulsed electromagnetic fields on the proliferation of rat marrow mesenchymal stem cells induced by 5-azacytidine. Methods Rat marrow mesenchymal stem cells induced for 7 d with 5-azacytidine were exposed or unexposed to 50 Hz pulsed electromagnetic fields and then randomly divided into A, B, C groups and 1, 2, 3, 4 subgroups according to different inductance intensity and different exposed time. Unexposed under pulsed electromagnetic fields was control groups. Proliferation capacity of rat marrow mesenchymal stem cells induced was examined by MTT method. Cell cycle was detected by flow cytometry. Results Under 0.5 mT and 1 mT inductance intensity for 20 - 30 min rat marrow mesenchymal stem cells induced by 5-azacytidine were proliferated significantly in comparison with control groups, while restrained significantly proliferation cell under 0.5 mT and 1 mT inductance intensity for 30 - 60 rain compared with control groups. Cell cycle at S ＋ G2 was increased under 0.5 mT and 1 mT inductance intensity for 20 - 30 min and decreased under 3 kinds inductance intensity for 60 min and 5 mT over 30 min versus control groups. Conclusion 50 Hz pulsed electromagnetic field at 0.5 mT and 1 mT inductance intensity for 20 - 30 min can promote the proliferation of mesenchymal stem cells by increasing cells at S and G2 stage.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2008年第5期421-423,共3页
Journal of Third Military Medical University
基金
国家自然科学基金(30370585)~~
关键词
脉冲电磁场
骨髓间充质干细胞
增殖
pulsed electromagnetic field： mesenchvmal stem cells, proliferation
