摘要
目的评估改良法制备异种脱蛋白骨（deproteinzated bone，DPB）作为组织工程支架材料修复大动物大段长骨缺损的能力，为异种DPB的临床应用提供实验依据。方法山羊24只，在每只山羊右侧胫骨中下段截除胫骨总长度20％形成节段性骨缺损，按分组情况植入不同材料。A组为单纯异种DPB，B组为自体骨，C组为异种DPB＋自体骨髓间充质干细胞（BMSCs）＋重组人骨形态发生蛋白2（rhBMP2），均采用半环槽式外固定器固定。术后4～24周每隔4周进行X线及术后24周取新生骨进行双能X线（DEXA）、组织学、生物力学检测，评价骨缺损修复效果。结果术后4～24周，在同一时相点，X线Lane—Sandhu法评分B组〉C组〉A组，并且术后24周新骨的骨密度、骨矿含量、组织学和生物力学检测经统计学分析，C组与B组比较，差异无统计学意义（P〉0．05）。结论改良法制备的异种DBP复合自体BMSCs和rhBMP2修复山羊胫骨大段缺损成骨能力与自体骨相当，可以作为组织工程支架材料试用于临床。
Objective To evaluate the ability of heterogeneous deproteinized bone （DPB） prepared by a modified method in repairing segmental long bone defects of big animals so as to provide experimental proof for clinical application of heterogeneous DPB. Methods A total of 24 goats were randomly divided into three groups, with eight goats in each group. Segmental bone defects of 20% right tibia middle and inferior diaphysis were prepared and implanted with DPB （Group A ）, autogenous bone （ Group B） and heterogeneous deproteinized bone/autogenous MSCs./rhBMP2 （ Group C） respectively and fixed by sulcated half-ring external fixator. X-ray examination was conducted every four weeks from 4 to 24 weeks postoperatively and dual energy X-ray absorptiometry （DEXA） , histological and vitodynamic examinations were conducted at 24 weeks postoperatively to detect the repair of the bone defects. Results The X-ray Lane score from 4 to 24 weeks was decreased from Group B to Group A. There was no statistical difference in bone density, mine contents, biomechanics and histology of the new bone at 24 weeks postoperatively between Groups B and C （ P 〉 0.05 ） Conclusions Heterogeneous DPB combined with autogenous MSCs/rhBMP2 has the same ability as autogenous bone to repair segmental long bone defects of the goat and can be applied clinically as a scaffold material for bone tissue engineering.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2008年第1期14-17,共4页
Chinese Journal of Trauma
基金
国家高技术研究发展计划重大攻关课题资助项目（2006AA02A122）
关键词
骨移植
异种
骨形态发生蛋白质类
组织工程
Bone transplantation, heterologous
Bone morphogenetic proteins
Tissue engineering
