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氨基酸对水牛孤雌激活胚胎体外发育的研究

Study on the Affects of Amino Acid on the Development of Buffslo Emtryos in Viyro
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摘要 本研究主要探讨氨基酸对水牛孤雌激活(PA)胚胎体外发育的影响。在SOF液中添加1.5%的MEM非必需氨基酸(NEAA)显著提高PA(79.8%vs 67.7%)胚胎的分裂率(P<0.05),囊胚发育率和囊胚孵化率无显著差异;添加1.0%的EAA显著提高PA胚胎的囊胚孵化率(77.3%vs 43.3%,P<0.05),但各组间的分裂率和囊胚发育率无显著差异;在改良SOF基础液中联合添加1.0%NEAA和2.0%EAA可显著提高孤雌激活胚胎的分裂率(84.4%vs 68.8%,P<0.05),但囊胚发育率和孵化率均无显著差异。 Effects of amino acids on the in vitro development of buffalo embryos derived from parthenogenetic activation （PA） were investigated, Addition of 1.5 % MEM nonessential amino acid solution（NEAA） to SOF medium resulted in a significant increase in the cleavage rate of PA （79.8% vs 67.7% ,P〈0.05）. However, supplementation with 3.0% BME essential amino acid solution （EAA） decreased the cleavage rate of IVF embryos （42.2% vs 52.2%, P〈0.05）, although the hatched rate of PA blastocysts was increased by supplementation with 1.0% EAA （77.3% vs 43.3% ,P〈0.05）.
作者 成俊萍 石德顺 陆风花 韦精卫 王晓丽 潘红平 李日聪
机构地区 广西人民医院不孕不育诊疗中心 广西大学动物繁殖研究所
出处 《畜牧兽医杂志》 2008年第1期1-3,共3页 Journal of Animal Science and Veterinary Medicine
基金 国家"863"高科技研究发展计划项目(批准号:2002AA206651)
关键词 水牛 孤雌激活 胚胎发育 氨基酸 buffalo parthenogenesis activation amino acid embryo development
分类号 S814.8 [农业科学—畜牧学]
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参考文献10

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二级参考文献15

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畜牧兽医杂志
2008年 第1期

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