摘要
研究了核糖胞嘧啶和脲嘧啶共聚物的酶促合成条件。共聚物ＰｏｌｙＣｘＵ与ＰｏｌｙＩ在类似生理盐水条件下形成错配的双链ＲＮＡ，然后对ＰｏｌｙＩ：Ｃ和错配的双链ＲＮＡ对核酸酶的敏感性及紫外熔化曲线进行了比较研究。
The enzymic synthesis conditions of Cytosine Uracil copolymers have been investigated. The equivalent molar weight mixtures of Poly CxU and Poly I in physiological saline like conditions formed the mismatched double stranded RNA. The comparable studies of Poly I:C and Poly I:CxU on the sensitive to nucleases and UV melting curves have shown that the sensitivity to nucleases for Poly I:CxU(in case of x>7) increased by 2-5 fold and the melting points decreased to 4-10℃ .A hypothesis that I and U bases occupied in the double stranded RNA could be in status wobble base pairs(I·U) has been suggested.
出处
《生物工程进展》
CSCD
1997年第4期14-18,共5页
Progress in Biotechnology
基金
北京市科学技术委员会重大科技项目
关键词
RNA
错配
双链RNA
稳性
Cytosine Uracil copolymer. Mismatched double stranded RNA. Sensitive to nucleases.UV melting curves. Wobble base pair.
