摘要
目的探讨己酮可可碱(PTX)对慢性充血性心力衰竭(CHF)患者外周血CD4^+辅助性T淋巴细胞(Th细胞)亚群平衡偏移的调节及意义。方法采用流式细胞分析法测定健康者(对照组,10例)、CHF患者(50例)加PTX(PTX组)及不加PTX(非PTX组)时的CD4^+Th细胞胞内细胞因子γ干扰素(IFN-γ)和白细胞介素(IL)-10。结果3组间IFN-γ、IL-10和IL-10/IFN-γ的差异均有统计学意义(F值分别=95.79、135.34和107.00,P值均<0.01)。PTX和非PTX组的IFN-γ均显著高于对照组(P<0.01),前两组间的差异也有统计学意义(P<0.01)。对照组的IL-10、IL-10/IFN-γ明显高于PTX和非PTX组(P值均<0.01),后两组间的差异也有统计学意义(P<0.01)。结论CHF患者存在CD4^+Th细胞亚群平衡偏移,而PTX可逆转充血性心力衰竭Th1/Th2细胞的失衡,从而调节CD4^+Th细胞的功能。
Objective To investigate the effect of pemoxifylline（PTX） on the imbalance of Th1/Th2 sub group in patients with chronic heart failure. Methods Peripheral blood cells were collected from 10 healthy subjects and 50 PTX-treated and non-treated patients with chronic heart failure. The levels of IFN-γ and IL-10 in CD4^＋ Th cells were determined by 3-color flow cytometry after stimulation with phorbol myristate acetate（PMA） and ionomycin. Results The levels of IFN-γ, IL-10 and the ratio of IL-10/IFN-γ were significantly different between the 3 groups（all P 〈 0.01）. The level of IFN-γ in CHF patients was significantly higher than that in healthy controls; there was also significant difference between PTX-treated and non-treated groups （P 〈 0.01）. The levels of IL-10 and the ratio of IL-10/IFN-γ in the control group were significantly higher than those of the PTX-treated and non-treated groups（both P 〈 0.01） ; there was also significant difference between PTX-treated and non-treated groups （P〈0.01）. Condusion Th1/Th2 functional imbalance exists in patients with CHF. PTX can correct the imbalance and subsequently regulate the function of CD4^＋ Th cells. （Shanghai Med J, 2007, 30：852-854）
出处
《上海医学》
CAS
CSCD
北大核心
2007年第11期852-854,共3页
Shanghai Medical Journal
