摘要
目的通过制造大鼠缺血模型,探讨缺血及再灌注后氧自由基清除剂依达拉奉(Edaravone)对大鼠脑皮质内谷氨酸含量的影响。方法线栓法制备局灶性脑缺血再灌注模型,分假手术组、盐水组、依达拉奉组,缺血30min再灌注120min,应用微透析技术观察大鼠脑内谷氨酸含量的变化。结果脑缺血后30min及再灌注20min盐水组和依达拉奉组皮质内谷氨酸含量有显著增加,以盐水组最明显;两者与假手术组比较差异均有极显著性意义(均P<0.01),其余时间点与假手术组比较差异无显著性意义(均P>0.05)。且依达拉奉组在以上时间内测得谷氨酸含量低于盐水组(P<0.05),其余时间点两者比较差异无显著性意义(均P>0.05)。结论依达拉奉对大鼠脑缺血再灌注致脑损伤有保护作用,其机制可能与拮抗氧自由基及抑制谷氨酸的释放有关。
Objective Using a rat model of middle cerebral artery occlusion and ischemia reperfusion（MCAO/IR） to inves tigate the effect of Edaravone on glutamlc acid contents of model rats. Methods Longa＇s method was employed to establish an animal MCAO/IR model. The dynamic changes of glutamic acid were observed by using microdialysis technique after Edaravone or saline was given respectively. Results Glutamic acid levels were increased obviously in saline group and edaravone group as compared with those in sham-operation group after 30 min of ischemia and the first 20 min of reperfusion（P〈0.01）. The levels of glutamic acid in edaravone group were notably lower than in the saline group 30 min after ischemia followed by 20 min reperfusion （P〈0.05）. No differences among these groups at other time points were observed（P〉0.05）. Conclusion Edaravone can effectively protect brain after cerebral ischemia-reperfusion injury, which may be related to the decreased release of glutamic acid.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2008年第1期114-116,共3页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
关键词
依达拉奉
谷氨酸
微透析
神经保护
Edaravone
glutamic acid
microdialysis
neuroprotective effect
