摘要
目的建立大鼠铜绿假单胞菌(PA)慢性肺部感染模型。方法健康Sprague-Dawley大鼠分为铜绿假单胞菌感染组与对照组。将铜绿假单胞菌包被的硅胶管(3Fr,直径1.0mm,长3.0mm)置入感染组大鼠的一侧主支气管处;对照组置入生理盐水浸泡的无菌硅胶管。接种后28d处死大鼠,分离肺组织,进行病理学检查和肺组织匀浆菌落计数。结果硅胶管置入后28d,感染组肺组织培养出野生型铜绿假单胞菌,匀浆菌落计数超过103cfu/g;大体病理表现为纤维增生、肉芽肿形成,镜下表现为淋巴细胞浸润为主的炎症反应;对照组大鼠肺组织未培养出铜绿假单胞菌,病理上未见明显改变。结论铜绿假单胞菌包被的硅胶管置入大鼠一侧主支气管,可成功建立慢性肺部感染模型。该方法造价低廉、可操作性较强,易于推广,为PA肺部感染药物治疗提供了依据。
Objective To establish a rat model of chronic pulmonary infection by inoculating Pseudomonas aeruginosa to Sprague-Dawley （SD） rats. Methods Sixty SD rats were divided into 2 groups, ie. the P. aeruginosa group and the control group. Silicone tube precoatod with P. aeruginosa was placed into the main bronchus. For the control group, sterile silicon tube was intubated. Results P. aeruginosa was detected from lung tissue of rats in infected groups. Bacterial number was higher than 10^3cfu/g 28 days after inoculation. The pathological study showed fibrinous proliferation and granulomas formation in the lungs of infected rats 28 days after inoculation. Microscopy examination showed a inflammation predominantly with lymphocyte infiltration. In control group, no bacterial and pathological changes could be detected. Condusion The animal model with P. aeruginosa chronic pulmonary infection can be established successfully by silicone tubes precoated with P. aeruginosa intubated into the main bronchus.
出处
《中国呼吸与危重监护杂志》
CAS
2008年第1期40-42,56,80,共5页
Chinese Journal of Respiratory and Critical Care Medicine
关键词
铜绿假单胞菌
慢性肺部感染模型
硅胶管
Pseudomonas aeruginosa
Chronic pulmonary infection model
Silicone tube
