摘要
目的评价日本血吸虫双价DNA疫苗的致突变性。方法应用Ames试验、小鼠骨髓多染红细胞微核试验、小鼠骨髓细胞染色体畸变试验对日本血吸虫双价DNA疫苗进行初步评价和检测。结果日本血吸虫双价DNA疫苗在S9活化和非活化两种测试条件下,在每皿6.25～100.00μg浓度范围内,对3种菌株的回复菌落数与阴性对照组比较,差异无统计学意义(P>0.05),Ames试验结果为阴性;日本血吸虫双价DNA疫苗各剂量组骨髓多染红细胞微核率与阴性对照组比较,差异无统计学意义(P>0.05),试验结果为阴性;日本血吸虫双价DNA疫苗各剂量组染色体畸变率与阴性对照组比较,差异无统计学意义(P>0.05),试验结果为阴性。结论日本血吸虫双价DNA疫苗初步检测无致突变性。
Objective To evaluate the mutagenicity of Schistosoma japonicum bivalent DNA vaccine. Methods The mutagenicity of Schistosoma japonicum bivalent DNA vaccine was studied by Ames test, micronucleus test and chromosome aberration test. Results On the conditions of actived and unactived, Schistosoma japonicum bivalent DNA vaccine did not lead obvious increase of colony units, and the result of the Ames test was negative. Compared with the control group, there were no significant differences in the treated groups on the frequency of micronucleus in PCE or chromosome aberration. Conclusion There is no mutagenicity of Schistosoma ja ponicum bivalent DNA vaccine.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2008年第1期37-40,共4页
Chinese Journal of Schistosomiasis Control
基金
国家高技术研究发展计划(863计划)(2004AA2Z3212)
