摘要
抗双链DNA（ds—DNA）抗体是系统性红斑狼疮（SLE）的特征性和致病性抗体，但该自身抗体的诱生机理至今不明。我们用刀豆蛋白（ConA）和脂多糖（LPS）活化的小鼠淋巴细胞为自身抗原，分别免疫同系小鼠，均能诱导出IgG类抗ds—DNA抗体，并且小鼠肾脏有IgG类免疫复合物沉积形成。结果提示活化淋巴细胞的核内成分是诱生抗ds—DNA抗体的自身抗原。
PURPOSE Anti - dsDNA autibodies are hallmark of SLE, but their autoantigen(s) was enigmatic. Weattempt to investigate the primary autoantigen(s) triggering the preduction of these pathogenic autoantitheies.METHODS We immunized nonautoimmune predisposed BALB/c mice with Con A activated, LPS activated and nonactivated syngeneic spleen cells. IgG anti - dsDNA were determined by ELISA. The immunecomplex in glomerulus was observed through immunofluoresecence.RESULTS Both Con A activated T lymphocytes and LPS activated B lymphocytes could induce IgG anti dsDNA antibodies in syngeneic nonautoimmune mice, but not nonactivate lymphocytes. Treated with glutaraldehyde could not influence the activated lyrnphocytes' ability to elicit the production of anti - DNA antibodies. Anti - DNA antibody positive mice had markedly immune complex deposits in glomerulus, which was analogous to spontaneous anti - DNA antibody in autoimmune mice.CONCLUSIONS Our results suggest that activated lymphocytes could be the autoimmunogen(s) drivingthe anti - dsDNA. autoantibodies.
出处
《上海医科大学学报》
CSCD
1997年第4期250-253,共4页
Journal of Fudan University(Medical Science)
基金
国家卫生部基金!94-1-162
