摘要
目的对河南汉族人群6个STR基因座的等位基因频率进行研究并获得群体遗传学数据。方法对121名无血缘关系河南汉族个体的EDTA抗凝血样用酚-氯仿法提取DNA,应用多重PCR扩增技术结合聚丙烯酰胺凝胶电泳对D2S1338、D4S2639、D7S3048、D8S1132、D14S306、D18S535共6个基因座在河南地区汉族人群中的基因型分布进行分析。结果6个STR基因座的基因型频率分布均符合Hardy-Weinberg平衡,各基因座的观察杂合度分别为0.843、0.842、0.857、0.900、0.824、0.826。结论6个基因座在河南汉族群体中具有较高的非父排除率和个体识别率,在法医学和群体遗传学研究中有一定的应用价值。
Objective To investigate the allele frequencies of six short tandem repeats （STR） loci D2S1338、D4S2639、D7S3048、D8S1132、D14S306、D18S535 in the HaM population of Henan province and to obtain preliminary data. Methods DNA was extracted with phenol - chloroform from 121 EDTA- blood specimens of healthy unrelated individuals in Henan population; multiplex PCR technique and PAGE vertical electrophoresis were used to screen the genotype frequencies of six STR systems in Henan population. Results The test for Hardy -Weinberg equilibrium revealed that the genotype distribution was correspondent with the expected. The observed heterozygosities of six loci were 0. 843,0. 842,0. 857,0. 900,0. 824,0. 826. Conclusion All of the six loci in this study have high power of discrimination and exclusion; they may be very useful genetic markers for individual identification, paternity test and genetics purposes.
出处
《医药论坛杂志》
2008年第2期13-14,共2页
Journal of Medical Forum
关键词
短串联重复序列
遗传多态性
河南汉族
Short tandem repeat
Genetic polymorphism
Han population of Henan province
