摘要
为进一步了解激素抵抗型哮喘的发病与细胞免疫功能紊乱的关系，应用地高辛标记ｃＤＮＡ探针、抗地高辛抗体－碱性磷酸酶检测系统行细胞涂片原位杂交，测定了１５例激素抵抗（ＳＲ）型和１５例激素敏感（ＳＳ）型哮喘患者外周血单个核细胞（ＰＢＭＣｓ）中白细胞介素（ＩＬ）－４和ＩＬ－５基因的ｍＲＮＡ表达。结果显示：（１）单独培养的ＳＲ型和ＳＳ型哮喘患者的外周血单个核细胞中ＩＬ－４ｍＲＮＡ和ＩＬ－５ｍＲＮＡ阳性细胞率差异无显著性（Ｐ＞０．０５）。（２）与氟美松（１０－７ｍｏｌ／Ｌ）共同培养４８小时后，ＳＳ型哮喘患者的外周血单个核细胞中ＩＬ－４ｍＲＮＡ和ＩＬ－５ｍＲＮＡ阳性细胞率较单独培养时明显减少（Ｐ＜０．０１）；而在ＳＲ型患者中则无明显改变（Ｐ＞０．０５）。提示氟美松不能有效地抑制ＳＲ型哮喘患者的外周血单个核细胞中ＩＬ－４和ＩＬ－５基因的ｍＲＮＡ表达，这可能是发生激素抵抗的机制之一。
The actual mechanism by which a group of asthmatics were resistant to glucocorticoids therapy remains poorly understood. Previous in vitro studies suggested that the pattern of cytokine gene expression may play a role in determining steroid resistance. In the current study, we used the technique of mRNA in situ hybridization to examine whether peripheral blood mononuclear cells (PBMCs) from steroid resistance (SR) asthmatics vs. Steroid sensitive (SS) asthmatics expressed different patterns of cytokines, particularly after treatment with steroid. PBMCs from SR patients did not differ significantly from those of SS in terms of their number expressing Interleukin(IL) 4 mRNA and IL 5 mRNA ( P >0.05) by culture alone. There were no differences in the number of PBMCs expressing IL 4 mRNA and IL 5 mRNA ( P >0.05) between incubation in the absence and presence of dexamethasone (DXM, 10 -7 mol/L) in SR. SS patients had a significant decrease in the number of PBMCs expressing IL 4 mRNA and IL 5 mRNA ( P <0.01) after culture in the presence of DXM as compared with incubation in media alone. Our findings indicate that SR asthma is associated with a dysregulation of the expression of the genes encoding for IL 4 and IL 5.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1997年第8期513-515,共3页
Chinese Journal of Internal Medicine
