摘要
为了检测脂蛋白（ａ）〔Ｌｐ（ａ）〕中的纤维蛋白溶酶原（Ｐｇ）位点，建立了用单克隆抗载脂蛋白（ａ）为包被抗体，酶标抗Ｐｇ为检测抗体的双抗体夹心酶联免疫吸附法。慢性肾衰（ＣＲＦ）患者Ｌｐ（ａ）中Ｐｇ位点、Ｐｇ／Ｌｐ（ａ）比值较对照组显著升高。提示ＣＲＦ患者体内Ｌｐ（ａ）发生氧化修饰、合成增加导致Ｌｐ（ａ）同Ｐｇ同源性增加，更具致动脉粥样硬化发生的危险性。
A “sandwich” ELISA for epitopes of plasminogen(Pg) in serum Lipoprotein(a) Lp(a) ,using monoclonal anti apo(a) as the capture antibody and quantitating with anti Pg enzyme conjuate had been developed.The results showed that chronic renal failure (CRF) patients had singnificantly elevated epitopes of Pg level、Pg/Lp(a) ratio in serum Lp(a).Oxidation of Lp(a) and increased synthesis might cause high Lp(a) more homologous to Pg,might be a more risk factor for development of atherosclerosis.
出处
《临床检验杂志》
CAS
CSCD
北大核心
1997年第4期199-202,共4页
Chinese Journal of Clinical Laboratory Science
