摘要
目的研究鳄鱼血浆对12种菌株的体外抗菌作用。方法采用平板菌落计数法,观察鳄鱼血浆与菌液作用1h后,对不同浓度细菌存活百分率的影响,从而测定鳄鱼血浆的抗菌范围;通过纸片法测定样品对各菌株的抑菌环直径,分析鳄鱼血浆的抗菌谱。结果与结论鳄鱼血浆对甲链球菌,丙链球菌,重白喉杆菌,肺炎双球菌,大肠杆菌M421C1ST,O111B4,E240-3LT,卡他球菌等8个菌株均显示出一定的抑菌作用。这可能是鳄鱼之间撕咬伤口不容易感染的原因,由此,鳄鱼血浆以及其它组织器官均有可能被开发成为天然抗菌药物。
Objective To investigate the effects of crocodilian plasma on 12 bacteria （including G＋bacteria and G- bacteria） lines in vitro. Methods The bacteria CFUs on nutrient broth agar plates were determined after bacteria and crocodilian plasma incubated together for lb. Then effects of the samples on the survival rate of various concentrations of bacteria were calculated. To evaluate the antibacterial spectrum of crocodilian plasma, the zones of bacteri- al inhibition were measured through agar disk method. Results and Conclusion Crocodilian plasma had superior bactericidal effects on Escherichia coli M421C1, OlllB4, E240-3 LT, Micrococcus catarrhalis , Shiga＇s bacillus, Pseudornonas aeruginosa and Diplococcus pneurnoniae. The results indicated why.wound of crocodilian by tearing each other was not susceptible,and crocodilian plasma could be developed a natural antibacterials.
出处
《中国海洋药物》
CAS
CSCD
2008年第1期33-39,共7页
Chinese Journal of Marine Drugs
基金
教育部2004年度"新世纪优秀人才支持计划"(NECT-04-0808)
霍英东教育基金会青年教师基金项目(91036)
国家海洋863重点项目(2006AA090404)
关键词
鳄鱼
血浆
抗菌
crocodilian
plasma
antibacterial
