摘要
采用静态吸附、解吸实验筛选6种树脂,确定选用AB-8树脂纯化肉桂总皂苷。动态吸附实验确定最佳工艺条件为上样pH值8～9,动态吸附流速1.0 mL/min,上样量为8倍树脂床体积,洗脱剂为65%乙醇溶液。HPLC分析证明纯化后的肉桂皂苷是含有多种同分异构体的混合物。
The equilibrium adsorption experiments and equilibrium desorption experiments were carried out by using six macroporous resins of different polarities to the crude saponins in cinnamon. The AB-8 resin was determined to purify the total saponins in cinnamon according to the experiment results. The optimum purification conditions confirmed by using dynamic adsorption experiment were as follows： the pH value of absorbing solution was 8-9, the absorbing velocity of flow was 1.0 mL/min, the proportion between the volume of absorbing solution and resins was 8：1, the concentration of ethanol was 65%. It was tested that the purified saponins in cinnamon was compound that contained several isomers by HPLC.
出处
《食品科技》
CAS
北大核心
2008年第2期199-202,共4页
Food Science and Technology
基金
黑龙江省研究生创新科研项目(YJSCX2007-0224HLJ)
